Genome editing for precision crop breeding

Part 1 of this volume reviews advances in gene editing techniques such as insertion-based genome edits, base editing, guide RNAs and CRISPR/Cas off targeting. Part 2 surveys applications of gene editing in key cereal and vegetable crops.

Part 1 Genome editing techniques1.Using TALENS and zinc finger nucleases as genome-editing reagents in plants: Adam Bogdanove, Cornell University, USA; 2.Double-strand break (DSB) repair in plants: Holger Puchta, Botanical Institute II - Karlsruhe Institute of Technology, Germany; 3.Advances in the generation of insertion-based genome edits in plants: Qing-Hui Yu, Xinjiang Chinese Academy of Agricultural Sciences, China; 4.Viruses as vectors for the delivery of gene-editing reagents: Evan E. Ellison, James C. Chamness and Daniel F. Voytas, University of Minnesota, USA; 5.Base editing in plants: Nathaniel Graham, Pairwise Plant, USA; 6.Advances in designing guide RNAs in genome editing of plants: Ling-Ling Chen, Huazhong Agricultural University, China; 7.Advances in assembling gRNA/Cas9 constructs in genome editing of plants: Diego Orzaez, Polytechnic University of Valencia, Spain; 8.Strategies for CRISPR/Cas9-mediated genome editing: from delivery to production of modified plants: William Gordon-Kamm, Pierluigi Barone, Sergei Svitashev, Jeffry D. Sander, Sandeep Kumar and Todd Jones, Corteva Agriscience, USA; 9.Advances in screening plants for edits and off targets: Chung Wang, Chinese Academy of Agricultural Sciences, China; 10.Genome editing on promoter regions: Andika Gunadi and Ning Zhang, Boyce Thompson Institute, USA; and John J. Finer, The Ohio State University, USA; 11.The regulation of genome-edited crops: Gregory Jaffe, Center for Science in the Public Interest, USA; Part 2 Applications12.Genome editing of barley: Martin Becker, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany; and Goetz Hensel, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany and Palacký University, Czech Republic; 13.Genome editing of maize: Kan Wang, Iowa State University, USA; 14.Genome editing of sorghum: David Holding, University of Nebraska-Lincoln, USA; 15.Genome editing of brassica crops: Cheng Dai, Huazhong Agricultural University, China; 16.Genome editing of tomatoes and other Solanaceae: Joyce Van Eck, Boyce Thompson Institute - Cornell University, USA; 17.Genome editing of perennial crops: Chung Jui (CJ) Tsai, University of Georgia, USA;