Plant Molecular Biology Manual - Supplement IV

Plant Molecular Biology Manual (Second Edition) is an entirely new manual containing both fundamental and recently described techniques in the area of plant molecular biology. Designed for use in the research laboratory, the Plant Molecular Biology Manual presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition.

Supplement I:
F4. Polymerase chain reaction: gene detection, inverse PCR, and genetic engineering; J. Slightom, et al. G3. Light microscopic applications of immunocytochemistry; K. Vandenbosch, et al. K3. T-DNA tagging in Arabidopsis thaliana: Cloning by gene disruption; B. Schulz, et al. L1. High level gene expression in plants using RNA viruses as transient expression vectors; C.M. Kearney, et al. L2. Geminivirus mediated gene transfer; D. Bisaro, G. Sunter. L3. Caulimovirus mediated gene transfer; I. Jupin, B. Gronenborn.
Supplement II:
RNA differential display; M.J. Laskowski. Expression analysis of multigene Families by RFLP-coupled domain-directed differential display (RC4D); A. Fischer, et al. Recombinant aequorin methods for measurement of intracellular calcium in plants; H. Knight, et al. GUS activity staining - A powerful tool in plant molecular biology; Jeong Hee Lee, F. Schöffl. In-Cell RT-PCR in a single, detached plant cell; Ho-Hyung Woo.
Supplement III:
A3. Liposome-mediated transfer of YAC-DNA to tobacco cells; M. Van Wordragen, et al. B7. Agribacterium germ-line transformation: Transformation of Arabidopsis without tissue culture; A.F. Bent, S. Clough. D6. RNA stability of chloroplasts; J. Nickelsen. G5. Preparation of extended DNA fibres for high resolution mapping by fluorescence in situ hybridization (FISH); P. Fransz, et al. L4. Tobamovirus transient expression vectors: Tools for plant biology and high-level expression of foreign proteins in plants; G.P. pogue, et al. M1. Identification and characterization of protein interactions using the yeast 2-hybrid system; S.E. Kohalmi, et al. Index.
Supplement IV:
List of Chapters. The construction of bacterial artificial chromosome (BAC) libraries; S. Choi, R.A. Wing. Yeast as a versatile tool in transcription factor research; A.H. Meijer, et al. Gene transfer mediated by site-specific recombination systems; L.A. Lyznik, et al. Use of the R/RS site-specific recombination system in plants; H. Albert, et al. Cre/Lox directed integration of transgenes into the tobacco genome; H. Albert, D.W. Ow. Plant actin isolation and characterization; C.J. Staiger, et al. Basic Work:
Section A: In vitro methods of gene transfer to plant cells. 1. PEG-mediated direct gene transfer and electroporation; R. Bilang, A. Klöti, M. Schrott, I. Potrykus. 2. Gene transfer to plants via particle bombardment; P. Christou. Section B: Agrobacterium-mediated gene transfer to plant cells. 1. Agrobacterium-mediated gene transfer to plant cells: Cointegrate and binary vectors; C.R. Walkerpeach, J. Velten. 2. Specialized vectors for gene tagging and expression studies; C. Koncz, N. Martini, L. Szabados, M. Hrouda, A. Bachmair, J. Schell. 3. Agrobacterium molecular genetics; P.J.J. Hooykaas. 4. Genetic manipulation of Agrobacterium tumefaciens strains to improve transformation of recalcitrant plant species; S.B. Gelvin, Chang-Nong Liu. 5. Transient expression assays using GUS constructs and fluorometric detection for analysis of T-DNA transfer; L.A. Castle, R.O. Morris. 6. Agrobacterium inoculation techniques for plant tissues; N.L. Mathis, M.A.W. Hinchee. Section C: Selectable and screenable markers for plant transformation. 1. Antibiotic resistance markers for plant transformation; M. van Montagu. 2. Reporter genes for plants; L. Herrera-Estrella, P. León, O. Olsson, T.H. Teeri. 3. Opines as screenable markers for plant transformation; Y. Dessaux, A. Petit. Section D: Nucleic acid extraction from plant tissue. 1. Extraction of total cellular DNA from plants, algae and fungi; S.O. Rogers, A.J. Bendich. 2. Isolation and characterization of nuclear scaffolds; G.E. Hall Jr., S. Spiker. 3. Isolation of plant mitochondria and mitochondrial nucleic acids; S.A. Mackenzie. 4. Isolation of chloroplasts and chloroplast DNA; C.A. Price, N. Hadjeb, L. Newman, E.M. Reardon. 5. Isolation of total, poly(A) and polysomal RNA; K. Pawlowski, R. Kunze, S. de Vries, T. Bisseling. Section E: Transcription and translation systems. 1. Assay for expression using run-on transcription in isolated nuclei; I.E. Somssich. 2. Preparation of an in vitro transcription system of plant origin, with methods and templates for assessing its fidelity; Y. Yamaguchi, F. Mukumoto, H. Imasehi, K-I. Yamazaki. Section F: Blotting and gene detection systems. 1. Southern, Northern and Western blot analysis; J. Memelink, K.M.M. Swords, L.A. Staehelin, J.H.C. Hoge. 2. Screening of cDNA expression libraries with synthetic oligonucleotides for DNA binding proteins; W. Werr, B. Uberlacker, B. Klinge. 3. Non-radioactive detection systems; S.J. Karcher. Section G: In situ hybridization and immunodetection. 1. RNA in situ hybridization in plants; N.B. Duck. 2. In situ hybridization to plant metaphase chromosomes using digoxigenin labeled nucleic acid sequences; S. Hinnisdaels, I. Farbos, J. Del-Favero, J. Veuskes, M. Jacobs, A. Mouras. Section H: Cloning and detection of DNA sequences from large DNA molecules. 1. Methods for generating plant genomic libraries; M.A. Snaed, P.L. Kretz, J.M. Short. 2. Construction of plant yeast artificial chromosome libraries; G.B. Martin. 3. Preparation of high molecular weight plant DNA and analysis by pulsed field gel electrophoresis; R.A.J.J. van Daelen, P. Zabel. 4. Random amplified polymorphic DNA (RAPD) markers; A. Rafalski, S. Tingey, J.G.K. Williams. Section I: Protein-nucleic acid interaction analyses. 1. Gel mobility shift assay; K. Mikami, H. Takase, M. Iwabuchi. 2. Optimilization of DNAse l footprinting experiments; S.J. Martino-Catt, S.A. Kay. 3. Analysis of plant chromatin and in vivo protein-DNA interactions; R.J. Ferl, A.-L. Paul. 4. Expression and characterization of recombinant plant transacting factors; E. Lam, L. Meisel. Section J: Subcellular targetting of proteins. 1. In vitro import of proteins into chloroplasts; B.D. Bruce, S. Perry, J. Froehlich, K. Keegstra. 2. In vitro targeting of proteins to mitochondria; M.A. Boutry, D. Thomas, F. Chaumont. 3. Targeting of proteins to the vacuole; J.D. Dombrowski, L. Gomez, M.J. Chrispeels, N.V. Raikhel. 4. Visualizing protein import into the plant cell nucleus; V. Citovsky. Section K: Gene tagging using transposons. 1. Gene tagging by endogenous transposons; W.-E. Lönnig, P. Huijser. 2. Heterologous transposon tagging as a tool for the isolation of plant genes; E.A. van der Biezen, M.J.J. van Haaren, B. Overduijn, H.J.J. Nijkamp, J. Hille. Index.