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Genotoxicity studies in D. melanogaster
Genotoxicity of Tryptophan and Sodium fluoride
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Artikel-Nr:
9783848446254
Einband:
.
Seiten:
92
Autor:
Priyanka Venugopal
Format:
22.00x15.00x0.50 cm
Sprache:
Englisch
Beschreibung:
Ms.V.Priyanka is a Post Graduate (M.Sc) in Human Genetics from Sri Ramachandra University,Chennai India. She is currently working as a Junior Executive in Sankara Nethralaya, Chennai,India. Her prime area of interest is Cancer biology. Her research work in Drosophila biology and Immunology has been published in Indian and International Journals.
The present study deals with the induction of mutations in D.melanogaster (Canton strain) by exposing them to various concentrations of Tryptophan and Sodium fluoride. The control flies and the flies exposed to the different concentrations of Tryptophan and Sodium fluoride were observed for phenotypic changes after 24hours and 48hours of exposure. The DNA was isolated from all the flies and the quantity and quality of the DNA were checked using Nanodrop and DNA fragmentation assay respectively. Shearing was observed in all the concentrations of Tryptophan and Sodium fluoride, by DNA fragmentation assay, which does not measure the total DNA damage. Therefore, advanced methods may be employed.