DNA Replication: The Regulatory Mechanisms

DNA replication is a key event in the cell cycle. Althoughour knowledge is far from complete and many elusiveregulatory mechanisms still remain beyondour grasp, manyenzymes and a multiplicity of biochemical mechanismsinvolved have been discovered. Recent findings in E. colihave confirmed and yet surpassed the original hypothesis ofF. Jacob. In yeast and higher eucaryotes, the apparentredundancy in putative origins and initiators has made anestimation of the importance of each identified elementdifficult to access. In spite of well establishedmethodologies - which are also described in the book - theorigin identification in mammalian chromosomes is still acontroversial subject. On the other hand, considerableadvances have been made in our understanding of virus DNAreplication and this continues to deepen and broaden ourunderstanding of the controls of cellular DNA replication.

Regulation of Initiation of Chromosome Replication.- 1: Cellular DNA Replication.- Cell cycle control of DNA replication.- 1. Escherichia coli strains in which the chromosome is replicated from origins other than oriC.- 2. Dam methyltransferase in Escherichia coli: Effects of different enzymatic levels on DNA replication and cell growth.- 3. Further characterization of a DNA replication inhibitor from the cell membrane of Escherichia coli.- 4. The involvement of cdc2 in cell cycle control of DNA replication in Xenopus egg extracts.- 5. A gene of Saccharomyces cerevisiae probably involved in the coordination of chromatin replication and cell division.- Origin identification in mammalian chromosomes.- 6. Origins of DNA replication and nucleosome segregation in mammalian chromosomes.- 7. Directions of DNA replication in yeast and mammalian cells.- 8. The curious case of the novel 2-D gel artifact.- Origin structure, function and modification.- 9. Structural and functional characteristics of autonomously replicating mammalian origin-enriched sequences (ORS).- 10. Mutations affecting replication origin function in yeast.- 11. Role of transcriptional elements in activating origins of replication.- 12. Telomere replication in Saccharomyces cerevisiae.- Initiator - replication origin recognition.- 13. The role of DnaA-DnaA box interaction in initiation of chromosomal replication in Bacillus subtilis.- 14. DnaA protein mediated transcription termination in the GUA operon of Escherichia coli.- 15. Screens for proteins binding to the ARS consensus sequence.- 16. Protein-DNA interaction at yeast replication origins: an ARS consensus binding protein.- 17. Genetic Analysis of the roles of yeast ARS binding factor I.- 18. Proteins involved in ARS function in yeast.- 19. ARS binding factors from Saccharomyces cerevisiae.- Initiator interactions with DNA polymerases and accessory proteins.- 20. Mutations within the minimal replicon of plasmid pPS10 increase its host range.- 21. Human type I DNA topoisomerase: Regulation of expression and function.- 22. Holoenzyme forms of yeast DNA polymerases ? and ?.- 23. The pol1 and pol3 DNA synthesis genes in fission yeast Schizosaccharomyces pombe.- 24. DNA Polymerase ? (POL3) of Saccharomyces cerevisiae.- 25. Genetic control of the DNA polymerase ?-primase complex in the yeast Saccharomyces Cerevisiae.- 2: Viral and Plasmid DNA Replication.- Molecular interactions during protein primed initiation.- 26. Protein-primed replication of bacteriophage Ø29 DNA.- 27. Molecular interactions at the adenovirus origin of DNA replication.- 28. Analysis in vitro of mutations in the cloned precursor to the terminal protein (pTP) and the adenovirus DNA polymerase (ad Pol) genes.- 29. The role of transcription factors in adenovirus DNA replication.- Molecular interactions at origins on circular viral and phage DNA.- 30. The replication initiator protein of P1 is activated by two E. coli heat shock proteins, DnaJ and DnaK.- 31. Bacteriophage Lambda DNA replication and the role of the universally conserved dnaK, dnaJ and grpE heat shock proteins.- 32. SV40 DNA replication with purified proteins : functional interactions among the initiation proteins.- 33. Bovine RP-A functions in SV40 DNA replication in vitro, but bovine polymerase ?-primase inhibits replication competitively.- 34. Bovine Papillomavirus (BPV1) replication protein E1: associated ATPase and binding to the viral E2 transactivator.