Enzyme Engineering

Enzyme Engineering An authoritative and up-to-date discussion of enzyme engineering and its applications In Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science, a team of distinguished researchers deliver a robust treatment of enzyme engineering and its applications in various fields such as biotechnology, life science, and synthesis. The book begins with an introduction to different protein engineering techniques, covers topics like gene mutagenesis methods for directed evolution and rational enzyme design. It includes industrial case studies of enzyme engineering with a focus on selectivity and activity. The authors also discuss new and innovative areas in the field, involving machine learning and artificial intelligence. It offers several insightful perspectives on the future of this work. Readers will also find: A thorough introduction to directed evolution and rational design as protein engineering techniques Comprehensive explorations of screening and selection techniques, gene mutagenesis methods in directed evolution, and guidelines for applying gene mutagenesis in organic chemistry, pharmaceutical applications, and biotechnology Practical discussions of protein engineering of enzyme robustness relevant to organic and pharmaceutical chemistry Treatments of artificial enzymes as promiscuous catalysts Various lessons learned from semi-rational and rational directed evolution A transdisciplinary treatise, Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science is perfect for protein engineers, theoreticians, organic, and pharmaceutical chemists as well as transition metal researchers in catalysis and biotechnologists.

Written by one of the pioneers, this book presents enzyme engineering and its applications at its best. Beside established topics like enzyme activity and selectivity, emerging fields like machine learning and AI are presented, too.

Introduction to Directed Evolution and Rational Design as Protein Engineering TechniquesMethods and Aims of Directed Enzyme EvolutionShort History of Directed Enzyme EvolutionMethods and Aims of Rational Design of EnzymesScreening and Selection TechniquesGene Mutagenesis MethodsGuidelines for Applying Gene Mutagenesis Methods in Organic Chemistry, Pharmaceutical Applications and BiotechnologyCase Studies of Protein Engineering of Activity and SelectivityEpoxide HydrolaseTransaminase as an Industrial Example with Pharmaceutical ApplicationGeranylgeranyl Diphosphate Synthase for Efficient Carotenoid ProductionCytochrome P450 Monooxygenases for Synthesis of Hydroxylation of Steroids Needed in the Preparation of PharmaceuticalsLipase for Stereocomplementary Production of Organic Compounds with Two Chirality CentersFurther Examples Using Other Enzyme TypesProtein Engineering of Enzyme RobustnessExamples of Relevance to Organic and Pharmaceutical ApplicationsExamples of Relevance to BiotechnologyArtificial Metallo-Enzymes for Promiscuous Transformations Using Known Organic Reaction Types as a GuideLearning Lessons from Protein EngineeringPerspectives for Future WorkIn Extending Applications in Organic and Pharmaceutical ChemistryIn Extending Biotechnological Contributions to Ecology