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Directed Evolution of Selective Enzymes
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Catalysts for Organic Chemistry and Biotechnology
E-Book
Sofort lieferbar | Lieferzeit: Sofort lieferbar
Artikel-Nr:
9783527655489
Veröffentl:
2016
Einband:
E-Book
Seiten:
320
Autor:
Manfred T. Reetz
eBook Typ:
EPUB
eBook Format:
Reflowable E-Book
Kopierschutz:
Adobe DRM [Hard-DRM]
Sprache:
Englisch
Beschreibung:
Authored by one of the world's leading organic chemists, this authoritative reference provides an overview of basic strategies in directed evolution and introduces common gene mutagenesis, screening and selection methods. Throughout the text, emphasis is placed on methodology development to maximize efficiency, reliability and speed of the experiments and to provide guidelines for efficient protein engineering. Professor Reetz highlights the application of directed evolution experiments to address limitations in the field of enzyme selectivity, substrate scope, activity and robustness. He critically reviews recent developments and case studies, takes a look at future applications in the field of organic synthesis, and concludes with lessons learned from previous experiments.
Authored by one of the world's leading organic chemists, this authoritative reference provides an overview of basic strategies in directed evolution and introduces common gene mutagenesis, screening and selection methods.Throughout the text, emphasis is placed on methodology development to maximize efficiency, reliability and speed of the experiments and to provide guidelines for efficient protein engineering. Professor Reetz highlights the application of directed evolution experiments to address limitations in the field of enzyme selectivity, substrate scope, activity and robustness. He critically reviews recent developments and case studies, takes a look at future applications in the field of organic synthesis, and concludes with lessons learned from previous experiments.
Introduction to directed evolutionSelection versus screening in directed evolutionGene mutagenesis methodsStrategies for applying gene mutagenesis methodsSelected examples of directed evolution of enzymes with emphasis on stereo- and regioselectivity, substrate scope and/or activityDirected evolution of enzyme robustnessDirected evolution of promiscuity: Artificial enzymes as catalysts in organic chemistryLearning from directed evolution