Handbook of RNA Biochemistry

The second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex. This broad scope allows the treatment of specialized methods side by side with basic biochemical techniques, making the book a real treasure trove for every researcher experimenting with RNA.

Ein umfassendes Kompendium für alle, die mit kleinen oder großen RNAs arbeiten. Bietet Hunderte von Protokollen, umfassende Erläuterungen und Tipps zur Fehlerbehebung, die in keinem anderen Nachschlagewerk zu finden sind.

RNA SYNTHESISEnyzmatic synthesis, ligation and modificationChemical synthesisNEW: RNA detection and quantitationSTRUCTURE DETERMINATIONSequence determinationNEW: Probing in vitro and in vivoFootprinting techniquesNucleotide analog interference mappingIdentification of interacting proteins and nucleic acidsSpectroscopic studies of RNA structureAtomic structure determinationNEW: Functional assaysNEW: Mass spectrometry of RNANEW: Folding and unfolding of single RNA moleculesRNA GENOMICS & BIOINFORMATICSPhylogenetic analysisStructure predictionModeling of RNA structuresMolecular dynamics simulationNEW: Searching and predicting small RNAsNEW: RNA mining and RNomics in eukaryotesNEW: Exon arraysANALYSIS OF RNA-PROTEIN INTERACTIONIsolation and purification of RNA-binding proteinsSedimentation analysis of RNP complexesProtein-RNA crosslinkingImmunoaffinity purificationNEW: TAP-tag affinity purificationThree-hybrid analysis to identify RNA-binding proteinsSELEXIn vitro selection of ribozymes and aptamersIn vivo SELEXIn vitro selection against small targetsNEW: Genomic SELEXRNA INTERFERENCENEW: Chemical modification of siRNANEW: siRNA design and deliveryNEW: RNAi in mammalian cells(partial contents)