Next Generation Genome Sequencing

Written by leading experts from industry and academia, this first single comprehensive resource addresses recent developments in next generation DNA sequencing technology and their impact on genome research, drug discovery and health care. As such, it presents a detailed comparative analysis of commercially available platforms as well as insights into alternative, emerging sequencing techniques. In addition, the book not only covers the principles of DNA sequencing techniques but also social, ethical and commercial aspects, the concept of personalized medicine and a five-year perspective of DNA sequencing.

Führende Experten aus der industriellen und akademischen Forschung fassen die neuesten Entwicklungen auf dem Gebiet der DNA-Sequenzierung zusammen und untersuchen, wie sich diese Neuerungen auf die Genomforschung, Wirkstoffentwicklung und das Gesundheitswesen (Stichwort: personalisierte Medizin) auswirken. Kommerziell verfügbare Plattformen werden ausführlich beschrieben und alternativen Verfahren gegenübergestellt. Neben den rein naturwissenschaftlich-medizinischen Fragen kommen auch soziale, ethische und wirtschaftliche Aspekte zur Sprache.

SANGER DNA SEQUENCINGSanger DNA sequencing (Men)NEXT GENERATION SEQUENCING - TOWARDS PERSONALIZED MEDICINEIllumina Genome Analyzer II System (Lakdawalla)Applied Biosystems SOLiD? System - Ligation-based Sequencing (Prediger)The next Generation Genome Sequencing: 454/Roche GS FLX (Egholm)Polony Sequencing - History, technology, and Applications (Edwards)THE BOTTLENECK: SEQUENCE DATA ANALYSISNext Generation Sequence Data Analysis (Bloksberg)DNASTARs Next Generation Software (Durfee)EMERGING SEQUENCING TECHNOLOGIESReal-time DNA sequencing (Hardin)Direct Sequencing by TEM of Z-substituted DNA Molecules (Glover)A Single DNA Molecule Barcoding Method with Applications in DNA Mapping and Molecular Haplotyping (Xao)Optical Sequencing - Acquisition from Mapped Single Molecule Templates (Schwartz)Microchip-based Sanger Sequencing of DNA (Barron)NEXT GENERATION SEQUENCING - TRULY INTEGRATED GENOME ANALYSISMultiplex Sequencing of Paired End diTags for Transcriptome and Genome Analysis (Ruan)Palaeogenomics using the 454 Sequencing Platform (Gilbert)ChIPSeq: Mapping of Protein-DNA Interactions (Fejes)MicroRNA Discovery and Expression Profiling using Next-generation Sequencing (Cuppen)DeepSAGE - Tag-based Transcriptome Analysis beyond Microarrays (Nielsen)The new Genomics and Personal Genome Information: Ethical Issues (Lunshof)