Infrared and Raman Spectroscopic Imaging

A comparison of the strengths and weaknesses of near-infrared, infrared and Raman imaging, focusing on current as well as conceivable applications for chemical analysis in delicate natural and synthetic samples. This handbook and ready reference covers instrumentation for vibrational spectroscopic imaging, chemometric evaluation of spectroscopic images, and vibrational spectroscopic imaging in biology and medicine, as well as the chemical, pharmaceutical and food industries.

Stärken, Nachteile und Einsatzmöglichkeiten bildgebender IR- und ramanspektroskopischer Verfahren untersuchen die Autoren dieses Handbuchs, das sich auch zum schnellen Nachschlagen in der Praxis eignet. Zu den Themen gehören die erforderliche instrumentelle Ausrüstung, die chemometrische Auswertung spektroskopisch gewonnener Bilder und natürlich zahlreiche Beispiele aus der Biologie, Medizin, Chemie, Pharmazie und Lebensmittelverarbeitung.

PART I: Basic MethodologyINFRARED AND RAMAN INSTRUMENTATION FOR MAPPING AND IMAGINGIntroduction to Mapping and ImagingMid-Infrared Microspectroscopy and MappingRaman Microspectroscopy and MappingNear-Infrared Hyperspectral ImagingRaman Hyperspectral ImagingMid-Infrared Hyperspectral ImagingMapping with Pulsed Terahertz RadiationCHEMOMETRIC TOOLS FOR IMAGE ANALYSISIntroductionHyperspectral Images: The MeasurementImage PreprocessingExploratory Image AnalysisQuantitative Image Information : Multivariat Image Regression (MIR)Image SegmentationImage ResolutionPART II: Biomedical ApplicationsVIBRATIONAL SPECTROSCOPIC IMAGING OF SOFT TISSUEIntroductionPreparation of Soft Tissue for Vibrational Spectroscopic ImagingApplications to Soft TissuesVIBRATIONAL SPECTROSCOPIC IMAGING OF HARD TISSUEInfrared and Raman Spectroscopy of BoneInfrared and Raman Imaging of BoneInfrared and Raman Spectroscopy of CartilageInfrared and Raman Imaging of CartilageINFRARED AND RAMAN SPECTROSCOPY AND SPECTRAL IMAGING OF INDIVIDUAL CELLSIntroductionMethodsResults and Discussion3-D IMAGING OF BIOMEDICAL SAMPLESIntroductionMethodologiesResultant 3-D ImagesPART III: Agricultural ApplicationsFT-IR MICROSPECTROSCOPIC IMAGING OF PLANT MATERIALIntroduction, Background and PerspectiveApplication of IMS to Kernels and Seeds of PlantsLeavesApplication of IMS to Stems and Cellulose PolymersAlgaeNEAR-INFRARED HYPERSPECTRAL IMAGING IN FOOD AND AGRICULTURAL SCIENCEIntroductionAt the Laboratory ScaleAt the Industrial ScalePART IV: Polymers and PharmaceuticalsFT-IR AND NIR SPECTROSCOPIC IMAGING: PRINCIPLES, PRACTICAL ASPECTS AND APPLICATIONS IN MATERIAL AND PHARMACEUTICAL SCIENCESIntroductionInstrumentation for FT-IR and NIR ImagingApplications of FT-IR Imaging for Polymer Research and Quality ControlNIR Imaging Spectroscopy for Quality Control of Pharmaceutical Drug FormulationsATR-FT-IR IMAGING FOR PHARMACEUTICAL AND POLYMERIC MATERIALS: FROM MICRO TO MACRO APPROACHESIntroductionThe Versatility of ATR-FT-IR ImagingApplications of ATR-FT-IR Imaging in the Materials SciencesApplications of ATR-FT-IR Imaging in the Pharmaceutical SciencesATR-FT-IR Imaging for Forensic ApplicationsRAMAN SPECTRAL IMAGING ON PHARMACEUTICAL PRODUCTSOverviewHyperspectral ImagingEmpirical Approach to Successful Raman Hyperspectral ImagingRaman in Multimodal Hyperspectral ImagingPART V: Developments in MethodologyDEEP, NONINVASIVE RAMAN SPECTROSCOPY OF DIFFUSELY SCATTERING MEDIAIntroductionTechniques for Deep, Noninvasive Raman SpectroscopyExamples of Application AreasOutlook on Raman TomographyPLANAR ARRAY INFRARED SPECTROSCOPY AND 1-D IMAGINGIntroductionConcept and InstrumentationPA-IR SpectroscopyApplicationsBIOMEDICAL APPLICATIONS OF INFRARED MICROSPECTROSCOPY USING SYNCHROTRON RADIATIONIntroductionSpatial Resolution ConsiderationsAdvantages of a Synchrotron IR SourceInstrumentationBiological Sample Preparation and Modes of Data CollectionBiological and Medical Applications on Synchrotron IR MicrospectroscopyFuture Directions for Synchrotron IR MicrospectroscopySPECTROSCOPIC IMAGING WITH NANOMETER RESOLUTION USING NEAR-FIELD METHODSIntroductionMethodsApplicationsCurrent Challenges