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Proteomics in Practice
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A Guide to Successful Experimental Design
E-Book
Sofort lieferbar | Lieferzeit: Sofort lieferbar
Artikel-Nr:
9783527622306
Veröffentl:
2008
Einband:
E-Book
Seiten:
502
Autor:
Reiner Westermeier
eBook Typ:
PDF
eBook Format:
Reflowable E-Book
Kopierschutz:
Adobe DRM [Hard-DRM]
Sprache:
Englisch
Beschreibung:
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended.Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
PrefaceForewordAbbreviationsGlossary of termsINTRODUCTIONHistoryCritical pointsHypothesis driven approach vs. High ThroughputComplementarities of workflowsConcept of experimental planningSuccess stories, highlights of useful referencesPART I: PROTEOMICS TECHNOLOGYElectrophoresisLiquid ChromatographyMass spectrometryBioinformatics tools / data miningMethods under developmentPART II. PRACTICAL MANUAL OF PROTEOME ANALYSISStrategy where to startGel-based workflowLC-based workflowLatest trendsValidation with Western blottingStatistic evaluationPART III: TROUBLE SHOOTINGGel-based workflowLC based workflowReferencesIndex