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Enzyme Assays
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High-throughput Screening, Genetic Selection and Fingerprinting
E-Book
Sofort lieferbar | Lieferzeit: Sofort lieferbar
Artikel-Nr:
9783527607211
Veröffentl:
2006
Einband:
E-Book
Seiten:
386
Autor:
Jean-Louis Reymond
eBook Typ:
PDF
eBook Format:
Reflowable E-Book
Kopierschutz:
Adobe DRM [Hard-DRM]
Sprache:
Englisch
Beschreibung:
Edited by one of the leading experts in the field, this book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years. For biochemists, analytical, organic and catalytic chemists, and biotechnologists.
Edited by one of the leading experts in the field, this book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years.For biochemists, analytical, organic and catalytic chemists, and biotechnologists.
PART I. THE CHEMISTRY OF ENZYME ASSAYS1. Chromogenic Assay of Hydrolases for Activity and Enantioselectivity2. High-throughput screening systems for assaying the enantioselectivity of enzymes3. High throughput screening methods developed for oxidoreductases4. Industrial perspective on assaysPART II. ENZYME ASSAYS AND GENETIC SELECTION5. Agar-plate based assays6. Genetic selection for biocatalysts7. Three-hybrid systems and in vivo selection8. Nucleic-acid based approaches on the screening of enzymes.PART III. ENZYME PROFILING9. Fluorescent probes for lipolytic enzymes10. Enzyme fingerprinting11. Protease profiling12. Enzyme assays on chipsINDEX