Protein Chromatography

Giorgio Carta received his PhD in Chemical Engineering from the University of Delaware in 1984. Since then he has been a professor in the Department of Chemical Engineering at the University of Virginia, Charlottesville (USA), where his research focuses on transport phenomena and bioseparations. He regularly organizes professional courses on various aspects of bioseparations, including a course on protein chromatography development and scale-up together with Alois Jungbauer.Alois Jungbauer is the head of protein technology and downstream processing at the Department of Biotechnology of the University of Natural Resources and Applied Life Sciences in Vienna (Austria). For more than 20 years, Professor Jungbauer has worked in biochemical engineering, with a focus on biosparation, where he has published widely and holds 15 patents. For over 10 years, he has organized a biennial professional course in protein chromatography focused on mass transfer, dispersion, and scale-up.

A practice-oriented all-in-one guide that provides the necessary knowledge for effective process development in chromatographic bioseparation, both for small-scale and large-scale applications.
With its focus on process development and bioseparation tasks, this is tailor-made reading for the professional bioengineer in both the biotech and pharmaceutical industries. Following a tried-and-tested concept, this guide has been developed over several years in training courses for biotech and chemical engineers in Europe and the U.S. The first part deals with the theory, introducing chromatography and its dynamics, as well as discussing mass transfer and dispersion effects. The second part then goes on to cover equipment and protocols, determining the retention factor and HETP from isocratic and elution experiments, as well as the mass transfer and intraparticle diffusivity from batch and shallow-bed adsorption experiments.

Ob kleiner oder großer Maßstab, hier finden Sie alles unter einem Dach: Praxisorientiert erklären die Autoren alles, was Sie über die chromatographische Bioseparation von Proteinen wissen müssen.

PrefaceDOWNSTREAM PROCESSING OF BIOTECHNOLOGY PRODUCTSIntroductionBioproducts and their ContaminantsBioprocessesRole of Chromatography in Downstream ProcessingReferencesINTRODUCTION TO PROTEIN CHROMATOGRAPHYIntroductionBasic Principles and DefinitionsModes of OperationPerformance FactorsSeparation Performance MetricsCHROMATOGRAPHY MEDIAIntroductionInteraction Types and ChemistryBuffers and Mobile PhasesPhysical Structure and PropertiesLABORATORY AND PROCESS COLUMNS AND EQUIPMENTIntroductionLaboratory-scale SystemsProcess Columns and EquipmentADSORPTION EQUILIBRIAIntroductionSingle Component SystemsMulti-component SystemsReferencesADSORPTION KINETICSIntroductionRate MechanismsBatch Adsorption KineticsDYNAMICS OF CHROMATOGRAPHY COLUMNSIntroductionConservation EquationsLocal Equilibrium DynamicsMulti-component SystemsDisplacement DevelopmentEFFECTS OF DISPERSION AND ADSORPTION KINETICS ON COLUMN PERFORMANCEIntroductionEmpirical Characterization of Column EfficiencyModeling and Prediction of Column EfficiencyGRADIENT ELUTION CHROMATOGRAPHYIntroductionGeneral Theory for Gradient Elution with Linear IsothermsLGE Relationships for Ion Exchange ChromatographyLGE Relationships for RPC and HICSeparations with pH GradientsModeling Gradient Elution with Non-linear IsothermsReferencesDESIGN OF CHROMATOGRAPHIC PROCESSESIntroductionChromatographic Process Steps and ConstraintsDesign for CaptureDesign for Chromatographic ResolutionSMB DesignReferencesINDEX