Beschreibung:
This handbook covers the most commonly used techniques for measuring plant response to biotic and abiotic stressing factors, including: in vitro and in vivo bioassays; the study of root morphology, photosynthesis (pigment content, net photosynthesis, respiration, fluorescence and thermoluminiscence) and water status; thermal imaging; the measurement of oxidative stress markers; flow cytometry for measuring cell cycle and other physiological parameters; the use of microscope techniques for studying plant microtubules; programmed-cell-death; last-generation techniques (metabolomics, proteomics, SAR/QSAR); hybridization methods; isotope techniques for plant and soil studies; and the measurement of detoxification pathways, volatiles, soil microorganisms, and computational biology.
This handbook covers the most commonly used techniques for measuring plant response to biotic and abiotic stressing factors, including: in vitro and in vivo bioassays; the study of root morphology, photosynthesis (pigment content, net photosynthesis, respiration, fluorescence and thermoluminiscence) and water status; thermal imaging; the measurement of oxidative stress markers; flow cytometry for measuring cell cycle and other physiological parameters; the use of microscope techniques for studying plant microtubules; programmed-cell-death; last-generation techniques (metabolomics, proteomics, SAR/QSAR); hybridization methods; isotope techniques for plant and soil studies; and the measurement of detoxification pathways, volatiles, soil microorganisms, and computational biology.
Preface.- 1. In vitro and in vivo bioassays.- 2. Root morphology.- 3. Plant photosynthetic pigments: Methods and tricks for correct quantification and identification.- 4. Measuring photosynthesis and respiration with infrared gas analysers.- 5. Chlorophyll fluorescence: a practical approach to study ecophysiology of green plants.- 6. Thermoluminescence: a tool to study ecophysiology of green plants.- 7. Determining plant water relations.- 8. Thermal imaging and infrared sensing in plant ecophysiology.- 9. Photoprotection and photo-oxidative stress markers as useful tools to unravel plant invasion success.- 10. Reactive oxygen species and antioxidant enzymatic systems in plants: role and methods.- 11. Flow cytometric measurement of different physiological parameters.- 12. Flow cytometry: cell cycle.- 13. Mitotic index.- 14. Fluorescent probes and live imaging of plant cells.- 15. Confocal and transmission electron microscopy for plant studies.- 16. Plant programmed cell death (PCD): using cell morphology as a tool to investigate plant PCD.- 17. Visualization of plant microtubules.- 18. Multiprobe in-situ hybridization to whole mount Arabidopsis seedlings.- 19. Proteomics analysis of plant tissues based on two-dimensional gel electrophoresis.- 20. Metabolomics and metabolic profiling – investigation of dynamic plant-environment interactions at the functional level.- 21. SAR / QSAR.- 22. Elucidating the phytotoxic potential of natural compounds.- 23. Exploring plants strategies for allelochemical detoxification.- 24. Chemical characterization of volatile organic compounds (VOCs) through headspace solid phase micro extraction (SPME).- 25. Carbon radiochemicals (14C) and stable isotopes (13C): crucial tools to study plant-soil interactions in ecosystems.- 26. Stable-isotope techniques to investigate sources of plant water.- 27. Soil microorganisms.- 28. Computational approach to study ecophysiology.
