Derivative Spectrophotometry and PAM-Fluorescence in Comparative Biochemistry

Introduction. Development of the methodological base, disputes, conclusions.- Successes of the pulse amplitude modulated fluorescence application: Fluorescence spectroscopy in amino acid analysis.- Theory of energetic bases of the green cells resistance to abiotic environmental factors.- Methodological approaches in our experimental work: The Main Law of Light Absorption by a Substance.- Correctness and Accuracy of Spectrophotometric Data.- Basis of Derivative Spectrophotometry.- Application of the ORIGIN package for processing of numerical spectra of biologically active substances and native structures.- New approaches for determination of oxygen isotopes in biochemical works.- Application of Derivative Spectrophotometry in Comparative Biochemical Studies: Analytical significance of derivative spectrophotometry in comparative studies of amino acids and proteins.- The derivative spectrophotometry for analysis of a number of guanidine preparations.- The proof of polycomponent composition of the promising antitumor drug "Ukrain".- Derived spectra application for the analysis of derived forms of nondepolarizing muscle relaxant tercuronium, of vitamins and hormones.- The Range of DHSO Application in Experiments with Pigments of Plants and Animals: New data on derived spectra of high orders for some carotenoids.- Neoxanthin as a probable key product of formation of - and beta- carotenoid derivatives.- Metabolic transformations of labeled 14C- or 3H- carotene in animal tissues.- Importance of the derivative spectrophotometry for study of alternative ways of carotenoids biosynthesis in Procaryota and Eucaryota.- Possibility of participation of -ketoglutaric acid funds in carotenoids biosynthesis in chloroplasts.- Malic acid as the source for carotenoids synthesis in plants with 4-way of carbon in photosynthesis.- Indication of the de-epoxidation reaction with help of derived spectra.- Conclusion.