Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice

Fatma Sarsu

Plant Breeding and Genetics Section

Joint FAO/IAEA Division

Vienna, Austria

Abdelbagi M.A. Ghanim

Plant Breeding and Genetics Laboratory

Joint FAO/IAEA Division

Vienna, Austria

Priyanka Das

School of Life Sciences

Jawaharlal Nehru University

New Delhi, India

Rajeev N Bahuguna

Jawaharlal Nehru University

New Delhi, India

Paul Mbogo Kusolwa

Sokoine University of Agriculture

Morogoro, United Republic of Tanzania

Muhammed Ashraf

Nuclear Institute for Agriculture and Biology (NIAB)

Faisalabad, Pakistan

Sneh Lata Singla-Pareek

International Centre for Genetic Engineering and Biotechnology

New Delhi, India

Ashwani Pareek

School of Life Sciences

Jawaharlal Nehru University

New Delhi, India

Brian P. Forster

Plant Breeding and Genetics Laboratory 

Joint FAO/IAEA Division

International Atomic Energy Agency 

Vienna, Austria

Ivan Ingelbrecht

Plant Breeding and Genetics Laboratory

Joint FAO/IAEA Division

International Atomic Energy Agency 

This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted - the seedling and flowering stages - with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly.

The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance.