Transgenic Insects

This book describes the huge opportunity to modify insect phenotypes through genetic engineering to benefit human health and agriculture. Precise DNA modifications and gene drive approaches are much more focused with improved safety. The development of modelling, ethical considerations, public response and regulatory oversight is covered.

Technology for modifying the genotypes and phenotypes of insects and other arthropods has steadily progressed with the development of more precise and powerful methods, most prominently transgenic modification. For many insect pests, there is now almost unlimited ability to modify phenotypes to benefit human health and agriculture. Precise DNA modifications and gene drive have the power to make wild-type populations less harmful in ways that could never have been performed with previous transgenic approaches. This transition from primarily laboratory science to greater application for field use has also necessitated greater development of modeling, ethical considerations and regulatory oversight. The 2nd Edition of Transgenic Insects contains chapters contributed by experts in the field that cover technologies and applications that are now possible. This edition includes increased attention to associated challenges of risk assessment, regulation, and public engagement. Featuring:Up-to-date analysis of molecular techniques, such as gene editing.Consideration of public attitudes and regulatory aspects associated with transgenic insects.Many examples of the wide range of applications of transgenic insects.This book will be very valuable to students and researchers in entomology, molecular biology, genetics, public health and agriculture, and will also appeal to practitioners who are implementing the technology, and to regulators, stakeholders and ethicists.

Transposon-Based Technologies for Germline Transformation of InsectsInducible / Repressible Systems for Transgene ExpressionSex, Tissue and Stage-specific Transgene ExpressionDocking Systems for Site-Directed Transgene IntegrationCRISPR Knock-in transformationReceptor-Mediated Ovary Transduction of Cargo (ReMOT Control)Site-directed DNA Sequence Modification Using CRISPR/Cas9Molecular Biology of Gene Drive SystemsDrosophila as a model to evaluate gene drive systemsGermline transformation of ticksTsetse Paratransgenesis: a Novel Strategy for Reducing the Spread of African TrypanosomiasisParatransgenesis of Glassy Winged SharpshooterParatransgenic Control of Chagas DiseaseAsaia Paratransgenesis of MosquitoesParatransgenesis in Mosquitoes and Other Insects: Microbial Ecology and Bacterial Genetic ConsiderationsRNA interferenceWolbachia transformationHoneybee transgenesisSex Ratio Manipulation Using Gene Drive for Mosquito Population ControlPopulation Alteration Using Gene Drive for Reduction of Malaria TransmissionUnderstanding Development Using Tribolium SilkwormTransgenesis and Applications Tephritid Fruit FlyTransgenesis and Applications Antiviral Effectors for MosquitoTransgenesis Conditional Dominant Lethals for population controlRIDL Transgenic Approaches for Sterile Insect Control of Dipteran Livestock Pests and Lepidopteran Crop Pests Acceptability of New Insect Vector Control TechnologiesRegulation of Transgenic InsectsEconomics of Transgenic Insects for Field ReleaseThe Cartagena Protocol Risk Analysis of Transgenic InsectsRIDL: Release of Insects Carrying a Dominant LethalModeling threshold-dependent gene drivesModeling self-limiting gene drives (killer-rescue, daisy chain)