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In-Cell NMR Spectroscopy
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From Molecular Sciences to Cell Biology
Besorgungstitel - wird vorgemerkt | Lieferzeit: Besorgungstitel - Lieferbar innerhalb von 10 Werktagen I
Artikel-Nr:
9781788012171
Veröffentl:
2019
Erscheinungsdatum:
13.12.2019
Seiten:
305
Autor:
Yutaka Ito
Gewicht:
635 g
Format:
236x163x25 mm
Sprache:
Englisch
Beschreibung:
In-cell NMR spectroscopy is a relatively new field. Despite its short history, recent in-cell NMR-related publications in major journals indicate that this method is receiving significant general attention. This book provides the first informative work specifically focused on in-cell NMR. It details the historical background of in-cell NMR, host cells for in-cell NMR studies, methods for in-cell biological techniques and NMR spectroscopy, applications, and future perspectives.
In-cell NMR Spectroscopy: From 2001 to Now; Protein NMR in Escherichia coli; In-cell NMR in Eukaryotic Cells - I (Introduction from Outside of Cells); In-cell NMR in Eukaryotic Cells - II (Direct Protein Expression); Protein Structure Determination in Living Cells from NOE-derived Distance Restraints; In-cell Structure Determination Using Paramagnetic NMR Spectroscopy; Bioreactor System for In-cell NMR Observation; 19F In-cell NMR; Cellular Solid-state NMR Spectroscopy; In-cell EPR; In-cell NMR: Spectral Optimization and Population Adjustment by Rational Protein Engineering; Protein Stability and Weak Intracellular Interactions; Protein Folding, Maturation and Redox State; All-atom Molecular Dynamics Simulation of Proteins in Crowded Environments; Primary Drug Screening by In-cell NMR Spectroscopy; In-cell NMR Spectroscopy of Nucleic Acids