Immunoproteomics

Providing current diverse approaches and techniques used to study the immunoproteome, Immunoproteomics: Methods and Protocols collects chapters from key researchers that deliver information to be used in diagnostics, disease progression, and vaccine correlates of protection analysis, to name but a few. This detailed volume includes techniques used for the study of the antibody targets of bacterial pathogens, viruses, and cancer, mass spectrometry-based approaches to characterize T-cell epitopes, chapters on detection and relative quantification of cytokines in serum, as well as in silico prediction of epitopes using sequence-based or modeling approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Immunoproteomics: Methods and Protocols aids researchers in transferring these techniques to their own laboratories in addition to providing a reference to guide researchers toward appropriate techniques.

Introduction to the Immune System.- Immunoproteomics: Current Technology and Applications.- Antigen Identification Using SEREX.- Antigen Discovery Using Whole-Genome Phage Display Libraries.- Methods and Applications of Serological Proteome Analysis.- Pre-Absorbed Immunoproteomics: A Novel Method for the Detection of Bacterial Surface Proteins.- Identification of the Antigen Content of Electroimmunoprecipitates.- An Immunoproteomics Approach to Screen the Antigenicity of the Influenza Virus.- In Vivo Microbial Antigen Discovery (InMAD) to Identify Diagnostic Proteins and Polysaccharides that Are Circulating During Microbial Infections.- Chemo-Enzymatic Production of O-Glycopeptides for the Detection of Serum Glycopeptide Antibodies.- Enrichment and Characterization of Glycopeptide Epitopes from Complex Mixtures.- Whole-Cell MALDI-TOF Mass Spectrometry: A Tool for Immune Cell Analysis and Characterization.- Cell-Based Arrays for the Identification of Interacting Polypeptide Domains or Epitopes.- Identification and Quantitation of MHC Class II-Bound Peptides from Mouse Spleen Dendritic Cells by Immunoprecipitation and Mass Spectrometry Analysis.- Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification.- Profiling of Cytokine and Chemokine Responses Using Multiplex Bead Array Technology.- Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis.- Identification of Protein Biomarkers in Human Serum Using iTRAQ and Shotgun Mass Spectrometry.- Genome-Based Bioinformatic Prediction of Major Histocompatibility Complex (MHC) Epitopes.- Structure-Based Prediction of Major Histocompatibility Complex (MHC) Epitopes.- Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell Interactomics.- Commercial Considerations for Immunoproteomics.