Lymphoma

Lymphomas are lymphoid malignancies derived from B or T lymphocytes, and their study has been and still is paradigmatic for many aspects of cancer research. Lymphoma: Methods and Protocols presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers. By covering a broad variety of methods used in lymphoma research, this book will be of interest not only for hematologists, hematopathologists, and immunologists but also for scientists interested in other fields of cancer research as well as human genetics. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Origin and Pathogenesis of B Cell Lymphomas.- Flow Cytometry for Non-Hodgkin and Classical Hodgkin Lymphoma.- Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells.- PCR-Based Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes by GeneScan Analysis or Heteroduplex Analysis for Clonality Assessment in Lymphoma Diagnostics.- Expression Cloning of Human B Cell Immunoglobulins.- Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR.- The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies.- Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas.- Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies.- MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR.- Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation.- Gene Expression Profile Analysis of Lymphomas.- FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas.- Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens.- Studying MicroRNAs in Lymphoma.- Molecular Methods of Virus Detection in Lymphoma.- High Throughput RNA Sequencing in B-Cell Lymphomas.Expression Cloning of Human B Cell Immunoglobulins.- Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR.- The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies.- Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas.- Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies.- MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR.- Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation.- Gene Expression Profile Analysis of Lymphomas.- FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas.- Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens.- Studying MicroRNAs in Lymphoma.- Molecular Methods of Virus Detection in Lymphoma.- High Throughput RNA Sequencing in B-Cell Lymphomas.