Spermiogenesis and Spermatogenesis
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Spermiogenesis and Spermatogenesis

Methods and Protocols
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Douglas T. Carrell
1259 g
260x183x36 mm
927, Methods in Molecular Biology

Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike.
This Methods in Molecular Biology(TM) book details protocols used in the study of spermatogenesis, including basic and advanced techniques used in clinical andrology laboratories. Includes materials lists, step-by-step protocols and troubleshooting tips.
Includes cutting-edge methods and protocols
Section I. Semen Analysis Methods

1. Methods For Sperm Concentration Determination

Lars Björndahl

2. Methods of Sperm Vitality Assessment

Sergey I. Moskovtsev and Clifford L. Librach

3. The Hypo-osmotic Swelling Test for Evaluation of Sperm Membrane Integrity

Sivakumar Ramu and Rajasingam S. Jeyendran

4. Sperm Morphology Classification: A Rational Method For Schemes Adopted By The World Health Organization

Susan A. Rothmann, Anna-Marie Bort, John Quigley, and Robin Pillow

5. Sperm Morphology Assessment Using Strict (Tygerberg) Criteria

Roelof Menkveld

6. Methods For Direct And Indirect Antisperm Antibody Testing

Hiroaki Shibahara and Junko Koriyama

7. Manual Methods For Sperm Motility Assessment

David Mortimer and Sharon T. Mortimer

8. Computer-Aided Sperm Analysis (CASA) Of Sperm Motility And Hyperactivation

David Mortimer and Sharon T. Mortimer

Section II. Tests of Sperm Function

9. The Hemizona Assay For Assessment Of Sperm Function

Sergio Oehninger, Mahmood Morshedi, and Daniel Franken

10. The Sperm Penetration Assay for the Assessment of Fertilization Capacity

Kathleen Hwang and Dolores J. Lamb

11. Methods For The Assessment Of Sperm Capacitation And Acrosome Reaction Excluding The Sperm Penetration Assay

Christopher J. De Jonge and Christopher L. R. Barratt

Section III. Sperm DNA Damage Testing

12. Sperm DNA Fragmentation Analysis Using the TUNEL Assay

Rakesh Sharma, Jayson Masaki, and Ashok Agarwal

13. Sperm DNA Damage Measured by Comet Assay

Luke Simon and Douglas T. Carrell

14. Sperm Chromatin Structure Assay (SCSA®)

Donald P. Evenson

Section IV. Advanced Clinical Testing

15. Sperm Aneuploidy Testing Using Fluorescence In Situ Hybridization (FISH)

Benjamin R. Emery

16. Flow Cytometric Methods For Sperm Assessment

Vanesa Robles and Felipe Martínez-Pastor

17. Human Y Chromosome Microdeletion Analysis By PCR Multiplex Protocols Identifying Only Clinically Relevant AZF Microdeletions

Peter H. Vogt and Ulrike Bender

Section V. Sperm Preparation and Selection Techniques

18. Sperm Cryopreservation Methods

Tiffany Justice and Greg Christensen

19. Density Gradient Separation Of Sperm For Artificial Insemination

David Mortimer and Sharon T. Mortimer

20. Recovery, Isolation, Identification and Preparation of Spermatozoa from Human Testis

Charles H. Muller and Erin R. Pagel

21. Enhancement of Sperm Motility Using Pentoxifylline and Platelet-Activating Factor

Shilo L. Archer and William E. Roudebush

22. Intracytoplasmic Morphology Selected Sperm Injection

Luke Simon, Aaron Wilcox, and Douglas T. Carrell

23. Sperm Selection For ICSI Using Annexin V

Sonja Grunewald and Uwe Paasch

24. Sperm Selection for ICSI Using the Hyaluronic Acid Binding Assay

Mohammad Hossein Nasr-Esfahani and Tavalaee Marziyeh

25. Sperm Selection Based On Electrostatic Charge

Luke Simon, Shao-qin Ge, and Douglas T. Carrell

26. Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting

Duane L. Garner, K. Michael Evans, and George E. Seidel, Jr.

Section VI. Staging and Histology Techniques

27. Assessment of Spermatogenesis Through Staging of Seminiferous Tubules

Marvin L. Meistrich and Rex A. Hess

28. Immunohistochemical Approaches for the Study of Spermatogenesis

Cathryn A. Hogarth and Michael D. Griswold

29. Ultrastructural Analysis of Testicular Tissue and Semen by Transmission and Scanning Electron Microscopy

Hector E. Chemes

Section VII. Oxidative Stress Testing

30. Assessment Of Oxidative Stress In Sperm And Semen

Anthony H. Kashou, Rakesh Sharma, and Ashok Agarwal

31. Improved Chemiluminescence Assay for Measuring Antioxidant Capacity of Seminal Plasma

Charles H. Muller, Tiffany K. Y. Tang, and Michalina A. Montaño

Section VIII. Analytical Tools and Methods

32. Methods of Sperm DNA Extraction for Genetic and Epigenetic Studies

Jeanine Griffin