Clinical Applications of Capillary Electrophoresis
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Clinical Applications of Capillary Electrophoresis

Methods and Protocols
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ISBN-13:
9781627030281
Erscheinungsdatum:
13.09.2012
Seiten:
311
Autor:
Terry M. Phillips
Gewicht:
816 g
Format:
260x183x21 mm
Serie:
919, Methods in Molecular Biology
Sprache:
Englisch
Beschreibung:

Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used. Clinical Applications of Capillary Electrophoresis aims to give an in-depth manual of CE applications in several important areas of clinical science. Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Clinical Applications of Capillary Electrophoresis seeks to serve as a valuable source of information not only for clinical pathologists, but also clinical scientists who wish to apply the technique to diagnosis and research.
This Methods in Molecular Biology(TM) book is an in-depth manual of Capillary Electrophoresis applications in important areas of clinical science, including clinical chemistry, hematology, disease-associated biomarker discovery, immunology and genetic analysis.
Includes cutting-edge methods and protocols
Part I. Overview

1. An Overview of CE in Clinical Analysis

David S. Hage

Part II. Applications in Clinical Chemistry

2. Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection

Petr Tuma and Karel Stulik

3. The Selective Determination of Sulfates, Sulfonates and Phosphates in Urine by Capillary Electrophoresis - Mass Spectrometry

Svenja-Catharina Bunz, and Christian Neusüß

4. Analysis of Neurotransmitter Amino Acids by CE-LIF Detection in Biological Fluids

Angelo Zinellu, Salvatore Sotgia, Luca Deiana, and Ciriaco Carru

5. Expanded Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)

Philip Britz-McKibbin

6. Development of an On-Site Measurement System for Salivary Stress-Related Substances Based on Microchip CE

Yoshihide Tanaka and Nahoko Naruishi

7. Simultaneous Determination of Atenolol and Amiloride by CZE with Capacitively Coupled Conductivity Detection (C4D)

Khaldun M. AL Azzam and Hassan Y. Aboul-Enein

8. A Homogeneous Immunoassay of Thyroxine Based on Microchip Electrophoresis and Chemiluminescence Detection

Shulin Zhao and Yi-Ming Liu

9. High-Throughput Profiling of the Serum N-Glycome on Capillary Electrophoresis Microfluidics Systems

Dieter Vanderschaeghe, Andras Guttman, and Nico Callewaert

10. Screening of Matrix Metalloproteinase Inhibitors by Microanalysis with Fluorescence Detection

Xin Hai, Erwin Adams, and Ann Van Schepdael

Part III. Applications in Hematology

11. ABO Genotyping by Capillary Electrophoresis

James Chun-I Lee, Hsing-Mei Hsieh, Hsiao-Feng Teng, and Li-Chin Tsai

12. Separation of Hemoglobin Variants by Capillary Electrophoresis

Frédéric Cotton and Béatric Gulbis

13. Newborn Screening for Hemoglobinopathies Using Capillary Electrophoresis

P.C. Giordano

Part IV. Applications in Bacteriology and Virology

14. Application of CZE in the Differentiation of Staphylococcus Aureus Strains

Boguslaw Buszewski, Katarzyna Hrynkiewicz, and Ewelina Dziubakiewicz

15. Multiplex and Quantitative Pathogen Detection with High-Resolution Capillary Electrophoresis-Based Single Strand Conformation Polymorphism

Hee Sung Hwang, Gi Won Shin, Boram Chung, Jeongkyeong Na, and Gyoo Yeol Jung

16. Application of CGE to Virus Identification

Julia A. Fruetel and Victoria A. VanderNoot

Part V. Applications in Disease Biomarker Discovery

17. Capillary Electrophoresis-Based Proteomic Techniques for Biomarker Discovery

Xueping Fang, Chenchen Wang, and Cheng S. Lee

18. Integrated Affinity and Electrophoresis Systems for Multiplexed Biomarker Analysis

Pamela N. Nge, Jayson V. Pagaduan, Weichun Yang, and Adam T. Woolley

19. Urinary Proteomics Based on Capillary Electrophoresis Coupled to Mass Spectrometry in Kidney Disease

Amaya Albalat, Julia Franke, Julien Gonzalez, Harald Mischak, and Petra Zürbig

20. Immunoaffinity, Capillary Electrophoresis, and Statistics for Studying Intact Alpha 1-Acid Glycoprotein Isoforms as Atherothrombosis Biomarker

Angel Puerta, Pedro J. Martin-Alvarez, Sara Ongay, Jose C. Diez-Masa and Mercedes de Frutos

Part VI. Applications in Immunology

21. Chip-Based Immunoassays

Akwasi A. Apori and Amy E. Herr

22. CE Analysis of g-Globulin Mobility and Potential Clinical Utility

Dieter Vanderschaeghe, Evi Debruyne, Hans Van Vlierberghe, Nico Callewaert, and Joris Delanghe

23. Cytokine Analysis by Immunoaffinity Capillary Electrophoresis

Mark Mendonca and Heather Kalish

Part VII. Applications in Clinical Genetic Analysis

24. Capillary Electrophoresis for the Detection of Fragile X Expanded Alleles

Rong Mao, Pinar Bayrak-Toydemir, and Elaine Lyon

25. Analysis of Microsatellite Instability by Microfluidic-Based Electrophoresis

Natalia Elfimova, Wafa Amer, and Margarete Odenthal

26. HLA DR-DQ Genotypi