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Methods and Protocols
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Robert Ménard
1390 g
260x183x37 mm
923, Methods in Molecular Biology

Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, 'omic' approaches, and techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium . Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Malaria: Methods and Protocols, Second Edition , offers a comprehensive set of standard techniques for laboratory researchers.
This Methods in Molecular Biology(TM) book expands on the thorough coverage of Malaria its previous edition, offering new chapters on parasite culture methods, genome manipulation and more, and offers materials lists, protocols and troubleshooting tips.
Presents definitive protocols for malaria researchers
'Part I. Parasite Culturing

1. In vitro Culturing P. falciparum Erythrocytic Stages

Alexander G. Maier and Melanie Rug

2. Production of Plasmodium falciparum Gametocytes in vitro

Kathryn Shaw Saliba and Marcelo Jacobs-Lorena

3. In vitro Differentiation of Plasmodium falciparum Gametocytes into Ookinetes

Anil K. Ghosh and Marcelo Jacobs-Lorena

4. Ex vivo culture of Plasmodium Vivax and Plasmodium Cynomolgi and in vitro Culture of Plasmodium Knowlesi Blood Stages

Anne-Marie Zeeman, Annemarie Voorberg-van der Wel, and Clemens H. M. Kocken

5. Laboratory Maintenance of Rodent Malaria Parasites

Chandra Ramakrishnan, Michael J. Delves, Kalpana Lal, Andrew M. Blagborough, Geoffrey Butcher, Kenneth W. Baker, and Robert E. Sinden

Part II. Genome Manipulation

6. Transfection of Plasmodium falciparum

Melanie Rug and Alexander G. Maier

7. Transfection of Rodent Malaria Parasites

Nisha Philip, Rachael Orr, and Andrew P. Waters

8. Recombination-mediated Genetic Engineering of Plasmodium berghei DNA

Claudia Pfander, Burcu Anar, Mathieu Brochet, Julian C. Rayner, and Oliver Billker

9. Standardization in Generating and Reporting Genetically Modified Rodent Malaria Parasites: The RMgmDB Database

Shahid M. Khan, Hans Kroeze, Blandine Franke-Fayard, and Chris J. Janse

10. Transient Transfection of Plasmodium vivax Blood-stage Parasites

Cecilia P. Sanchez, Judith Pfahler, Hernando A del Portillo, and Michael Lanzer

11. Silencing of Genes and Alleles by RNAi in Anopheles gambiae

Marina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, and Stéphanie A. Blandin

Part III. 'OMIC' Techniques

12. Analysis of Variant Gene Family Expression by Quantitative PCR

Sohini Sanyal, Thomas J. Templeton, and Cristina K. Moreira

13. DNA Microarray-based Genome-wide Analyses of Plasmodium Parasites

Zbynek Bozdech, Sachel Mok, and Archna P. Gupta

14. Whole-genome Analysis of Plasmodium spp . Utilizing a New Agilent Technologies DNA Microarray Platform

Heather J. Painter, Lindsey M. Altenhofen, Björn F.C. Kafsack and Manuel Llinás

15. Transcriptome Analysis using RNA-seq

Wieteke A.M. Hoeijmakers, Richárd Bártfai, and Hendrik G. Stunnenberg

16. Experimental Tools for the Study of Protein Phosphorylation in Plasmodium

Dominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, and Christian Doerig

17. Extraction of Hydrophilic Metabolites from Plasmodium falciparum -infected Erythrocytes for Metabolomic Analysis

Kellen L. Olszewski and Manuel Llinás

Part IV. Red Blood Cell Stages

18. Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparum

David T. Riglar and Jake Baum

19. Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparum Merozoites

Shailja Singh and Chetan E. Chitnis

20. Microsphiltration: a Microsphere Matrix to Explore Erythrocyte Deformability

Catherine Lavazec, Guillaume Deplaine, Innocent Safeukui, Sylvie Perrot, Geneviève Milon, Odile Mercereau-Puijalon, Peter H. David, and Pierre Buffet

21. Atomic Force Microscopy of Plasmodium -infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition Events

Ang Li, Laurent Rénia, Chwee Teck Lim, and Bruce Russell

22. Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System

Anand Srivastava, Yves Durocher, and Benoît Gamain

23. Genome-wide Chromatin Immunoprecipitation-sequencing in Plasmodium

Jose-Juan Lopez-Rubio, T. Nicolai Siegel, and Artur Scherf

24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparum

Liliana Mancio-Silva and Artur Scherf

25. Bioluminescence Imaging of P. berghei Schizont Sequestration in Rodents

Joanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Ch