Genomic Imprinting
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Genomic Imprinting

Methods and Protocols
 Book
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ISBN-13:
9781627030106
Einband:
Book
Erscheinungsdatum:
01.09.2012
Seiten:
297
Autor:
Nora Engel
Gewicht:
787 g
Format:
260x183x21 mm
Serie:
925, Methods in Molecular Biology
Sprache:
Englisch
Beschreibung:

Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In Genomic Imprinting: Methods and Protocols , experts in the field provide a survey of the technologies that are being applied to advance the study of imprinting. This detailed volume features new technologies that are accelerating the pace of discovery of imprinted genes and characterization of their epigenetic profile, bioinformatic procedures for prediction and comparative analyses of imprinted genes, as well as methods in embryology and basic molecular biology that have been employed for many years, some appearing in new versions for small cell numbers. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Genomic Imprinting: Methods and Protocols will aid scientists in unveiling both much awaited answers and all-new questions to keep this vital field busy for many exciting years to come.
This Methods in Molecular Biology(TM) book surveys technologies that are advancing the study of imprinting, viewing new technologies that are accelerating the pace of discovery of imprinted genes. Includes materials lists, protocols and troubleshooting tips.
Surveys cutting-edge technologies applied in the field
Part I: Parent-of-Origin Effects

1. Uniparental Embryos in the Study of Genomic Imprinting

Yong Cheng, Dasari Amarnath, and Keith E. Latham

2. Derivation of Induced Pluripotent Stem Cells by Retroviral Gene Transduction in Mammalian Species

Masanori Imamura, Hironobu Okuno, Ikuo Tomioka, Yoshimi Kawamura, Zachary Yu-Ching Lin, Ryusuke Nakajima, Wado Akamatsu, Hirotaka James Okano, Yumi Matsuzaki, Erika Sasaki, and Hideyuki Okano

3. Generation of Trophoblast Stem Cells

Michael C. Golding

4. Immunomagnetic Purification of Murine Primordial Germ Cells

Emily Y. Smith and James L. Resnick

Part II: Identifying Imprinted Genes

5. Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA

Andrew J. Sharp

6. Identification of Imprinted Loci by Transcriptome Sequencing

Tomas Babak

7. Data Mining as a Discovery Tool for Imprinted Genes

Chelsea Brideau and Paul Soloway

Part III: Identifying the Regulatory Features of Imprinted Domains

8. Engineering of Large Deletions and Duplications In Vivo

Louis Lefebvre

Part IV: Epigenetics of Imprinted Regions

9. Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells

Julie Borgel, Sylvain Guibert, and Michael Weber

10. Chromatin Immunoprecipitation to Characterize the Epigenetic Profiles of Imprinted Domains

Purnima Singh and Piroska E. Szabó

11. Quantitative Chromosome Conformation Capture
Raffaella Nativio, Yoko Ito, and Adele Murrell

12. Genome-Wide Analysis of DNA Methylation in Low Cell Numbers by Reduced Representation Bisulfite Sequencing
Sébastien A. Smallwood and Gavin Kelsey

Part V: Analysis of Imprinted Expression

13. Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytes for Imprinting Studies

Sarah Rose Huffman, Md Almamun, and Rocío Melissa Rivera

14. Generation of cDNA Libraries from RNP-Derived Regulatory Non-Coding RNAs

Mathieu Rederstorff

15. Co-Immunoprecipitation of Long Non-Coding RNAs

Victoria A. Moran, Courtney N. Niland, and Ahmad M. Khalil

Part VI: Imprinting in Plants

16. Specialized Technologies for Epigenetics in Plants

Wenyan Xiao

Part VII: Evolution of Imprinted G enes

17. Computational Studies of Imprinted Genes

Martina Paulsen

18. Insights on Imprinting from Beyond Mice and Men

Andrew Pask

19. Non-Mammalian Parent-Of-Origin Effects

Elena de la Casa-Esperón

14. Generation of cDNA Libraries from RNP-Derived Regulatory Non-Coding RNAs

Mathieu Rederstorff

15. Co-Immunoprecipitation of Long Non-Coding RNAs

Victoria A. Moran, Courtney N. Niland, and Ahmad M. Khalil

Part VI: Imprinting in Plants

16. Specialized Technologies for Epigenetics in Plants

Wenyan Xiao

Part VII: Evolution of Imprinted G enes

17. Computational Studies of Imprinted Genes

Martina Paulsen

18. Insights on Imprinting from Beyond Mice and Men

Andrew Pask

19. Non-Mammalian Parent-Of-Origin Effects

Elena de la Casa-Esperón

14. Generation of cDNA Libraries from RNP-Derived Regulatory Non-Coding RNAs

Mathieu Rederstorff

15. Co-Immunoprecipitation of Long Non-Coding RNAs

Victoria A. Moran, Courtney N. Niland, and Ahmad M. Khalil

Part VI: Imprinting in Plants

16. Specialized Technologies for Epigenetics in Plants

Wenyan Xiao

Part VII: Evolution of Imprinted G enes

17. Computational Studies of Imprinted Genes

Martina Paulsen

18. Insights on Imprinting from Beyond Mice and Men

Andrew Pask

19. Non-Mammalian Parent-Of-Origin Effects

Elena de la Casa-Esperón

Raffaella Nativio, Yoko Ito, and Adele Murrell

12. Genome-Wide Analysis of DNA Methylation in Low Cell Numbers by Reduced Representation Bisulfite Sequencing
Sébastien A. Smallwood and Gavin Kelsey

Part V: Analysis of Imprinted Expression

13. Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytes for Imprinting Stud