SELDI-TOF Mass Spectrometry

Coupling features of chromatography and mass spectrometry, SELDI is a popular method of biomarker discovery. This volume reviews its current applications, emphasizing study and experimental design, data analysis and interpretation, and assay development.

SELDI is distinct from other TOF-MS technologies in that it couples features of chromatography and mass spectrometry, facilitating analyte enrichment and sample cleanup on an array surface. In the growing field of proteomics, SELDI technology has been widely used for biomarker discovery and characterization in diverse applications including diagnostics, drug development, and basic research. SELDI-based biomarker studies can typically be divided into four phases: discovery, validation, purification and identification, and assay development. SELDI-TOF Mass Spectrometry: Methods and Protocols provides an overview of the current applications of SELDI-TOF MS (surface enhanced laser desorption/ionization time-of-flight mass spectrometry), with an emphasis on study and experimental design, data analysis and interpretation, and assay development. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and accessible, SELDI-TOF Mass Spectrometry: Methods and Protocols will provide information on optimizing study design, experimental protocols, and data analysis and interpretation to yield robust biomarkers and biomarker assays, using examples from different disease areas.

1. Optimized Conditions for a Quantitative SELDI TOF MS Protein AssayLee Lomas, Charlotte Clarke, Vanitha Thulasiraman, and Eric Fung2. Solid-phase Fractionation Strategies Applied to Proteomics InvestigationsLuc Guerrier, Frederic Fortis, and Egisto Boschetti3. Data Processing and Analysis Using ProteinChip® Data Manager SoftwareEnrique A. Dalmasso and Dominic Caseñas4. Purification and Identification of Candidate biomarkers discovered using SELDI TOF MSAmanda L. Bulman and Enrique Dalmasso5. Biomarker Discovery in Serum/Plasma Using Surface Enhanced Laser Desorption Ionization Time Of Flight (SELDI TOF) Mass Spectrometry PlatformMomar Ndao6. Plasma Proteomic Profiling of Pediatric OsteosarcomaYiting Li, TuAhn Dang, and Tsz-Kwong Man7. Profiling of Urine Using ProteinChip TechnologyRonald L. Woodbury, Diane B. McCarthy, and Amanda L. Bulman8. Protein Profiling of Cerebrospinal FluidAnja H. Simonsen9. SELDI TOF Mass Spectrometry-Based Protein Profiling of Tissue Samples for Toxicological StudiesAlexandra Sposny and Philip G. Hewitt10. Proteomic Analysis of Skeletal Muscle Tissue Using SELDI TOF MS: Application to Disuse AtrophyMark S. F. Clarke11. Profiling Cervical Lavage Fluid by SELDI-TOF Mass SpectrometryAdam Burgener12. Isolation and Proteomic Analysis of Platelets by SELDI TOF MSSean R. Downing and Giannoula L. Klement13. Using SELDI-TOF Mass spectrometry on Amniotic Fluid and for Clinical Proteomics and Theranostics in Disorders of PregnancyIrina A. Buhimschi14. High Throughput Profiling of Serum Phosphoproteins/ peptides using the SELDI TOF MS PlatformLin Ji, Gitanjali Jayachandran and Jack A. Roth15. Analysis of Protein-protein Interaction using ProteinChip Array- based SELDI TOF Mass SpectrometryGitanjali Jayachandran, Jack A. Roth, and Lin Ji16. Quantitation of Amyloid Beta Peptides in CSF by Surface Enhanced MALDI-TOFEddie Takahashi, Anita Howe, Ole Vesterqvist and Zhaosheng Lin