Immunoproteomics
Beschreibung:
Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhancedhands-on insights into this evolving field.
Introduction to the Immune System.- Immunoproteomics Methods and Techniques.- Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification.- Profiling of Cytokine and Chemokine Responses using Multiplex Bead Array Technology.- Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis.- Enriching for Low Abundance Serum Proteins using ProteoMiner(TM) and Protein Level HPLC.- Identification of the Antigen Content of Electroimmunoprecipitates.- Detection of Human Norovirus-Specific Antibodies using the Luciferase Immunoprecipitation System (LIPS) Assay.- Intact Mass Spectrometry Analysis of Immuno-Isolated Human Therapeutic Antibodies from Serum.- Antigen Identification for Cell-Binding Antibodies using Ligand Directed Crosslinking and Biotin Transfer.- Whole Genome-Phage Display Libraries: A Powerful Tool for Antigen Discovery.- Multiplexed Detection of Autoantibodies to Glycopeptides using Microarray.- Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification.- Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity Purification.- Enhanced Mass Spectrometry Detection of MHC Peptides.- Enrichment of Phosphorylated MHC Peptides with Immobilized Metal Affinity Chromatography and Titanium Dioxide Particles.- Mass Spectrometric Identification and Molecular Modeling of Glycopeptides Presented by MHC Class I and II Processing Pathways.- Genome Based Bioinformatic Prediction of Major Histocompatibility (MHC).- Enhancing Mass Spectrometry-Based MHC-I Peptide Identification through a Targeted Database Search Approach.- Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell Interactomics.- Genomics Driven Immunoproteomics: An Integrative Platform to Uncover Important Biomarkers for Human Diseases.- Identification of Antigens in Immune Complexes.- Analysis and Characterization of Immune Cells and their Activation Status by Whole-Cell MALDI TOF Mass Spectrometry.- A Molecular Immunoproteomics Approach to Assess the Viral Antigenicity of Influenza.- Immunoproteomic Biomarkers: From Publication to Personalized Medicine.