Lymphoma

This second edition volume expands on the previous edition with descriptions of recent developments in the field. The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by a proximity ligation assay, and detection of circulating cell-free tumor DNA in lymphomas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Innovative and thorough, Lymphoma: Methods and Protocols, Second Edition is a valuable resource for hematologists, hematopathologists, and any scientists interested in cancer research, human genetics, and immunology.

Includes cutting-edge methods and protocols

Origin and Pathogenesis of B Cell Lymphomas.- Flow Cytometry for Non-Hodgkin and Hodgkin Lymphoma.- Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells.- PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations.- Expression Cloning of Antibodies from Single Human B Cells.- Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR.- Stereotyped B Cell Immunoglobulins in B Cell Lymphomas.- Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies.- MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR.- Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA Input.- FISH and FICTION in Lymphoma Research.- NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma.- RNA Sequencing in B-Cell Lymphomas.- Studying Cancer Heterogeneity by Single Cell RNA-Sequencing.- Evaluation of Whole Genome Sequencing Data.- Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines.- Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens.- Proximity Ligation Assay.- Phospoproteomic Analysis of Signaling Pathways in Lymphomas.- Ultrasensitive Detection of Circulating Tumour DNA in Lymphoma via Targeted Hybridization Capture and Deep Sequencing of Barcoded Libraries.