Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease.- Purification of Basophils from Peripheral Human Blood.- Mast Cell Purification Protocols.- Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells.- Generation of Mast Cells from Murine Stem Cell Progenitors.- Integration of the Human Dermal Mast Cell into the Organotypic Coculture Skin Model.- The Absolute Basophil Count.- Detection of Mast Cells and Basophils by Immunohistochemistry.- Mast Cell and Basophil Cell Lines: A Compendium.- Measuring Histamine and Cytokine Release from Basophils and Mast Cells.- Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques.- Microscopy Assays for Evaluation of Mast Cell Migration and Chemotaxis.- Use of Humanized Rat Basophil Leukaemia (RBL) Reporter Systems for Detection of Allergen-Specific IgE Sensitization in Human Serum.- Gene Silencing Approaches in Mast Cells and Primary Human Basophils.- Basophil Stimulation and Signaling Pathways.- Identification and Immunophenotypic Characterization of Normal and Pathological Mast Cells.- Identification of Murine Basophils by Flow Cytometry and Histology.- Mast Cell Mediated Reactions In Vivo.