Chromosome Architecture

This volume details a valuable collection of protocols andreviews, such as emerging experimental and theoretical approaches. Theseapproaches have resulted in a substantial improvement in the understanding ofchromosome architecture. Chromosome Architecture:Methods and Protocols guides readers through cutting-edge interdisciplinarymethods which allow for an understanding of architecture of chromosomes withexceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.

New Advances in Chromosome Architecture.- Single MoleculeNarrowfield Microscopy Of Protein-DNA Binding Dynamics In Glucose SignalTransduction Of Live Yeast Cells.- Single-Molecule Imaging to Characterise the TransportMechanism of the Nuclear Pore Complex.- Using Fluorescence Recovery after Photobleaching (FRAP)To Study Dynamics Of The Structural Maintenance Of Chromosome (SMC) Complex InVivo.- Imaging DNA Structure by Atomic Force Microscopy.- Investigating Bacterial ChromosomeArchitecture Using Epifluorescence Microscopy.- Transverse Magnetic TweezersAllowing Coincident Epi-Fluorescence Microscopy On Horizontally Extended DNA.- Probing Chromosome Dynamics in Bacillus Subtilis.- Systems Biology Approaches for Understanding Genome Architecture.- InVivo And In Situ Replication Labeling Methods For Super-ResolutionStructured Illumination Microscopy Of Chromosome Territories And ChromatinDomains.- DNA-Protein InteractionsStudied Directly Using Single Molecule Fluorescence Imaging Of Quantum DotTagged Proteins Moving On DNA Tightropes.- Escherichia Coli Chromosome CopyNumber Measurement Using Flow Cytometry Analysis.- Bacterial Chromosome Dynamics by Locus Tracking In FluorescenceMicroscopy.- Biophysical Characterization of Chromatin Remodelling Protein CHD4.- Atomistic MolecularDynamics Simulations of DNA Minicircle Topoisomers: A Practical Guide to Setup,Performance and Analysis.- Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells.- Studying the Dynamics of Chromatin Binding Proteins in Mammalian CellsUsing Single-Molecule Localization Microscopy.- IntraNuclear Single Particle Tracking (I-SPT) To Reveal the Functional Architecture OfChromosomes.- Visualizing Bacillus Subtilis during Vegetative Growth and Spore Formation.