Sperm Chromatin

Sperm DNA damage is common and has been associated with reduced rates of conception, impaired embryonic development and increased risk of miscarriage. Although the exact causes of sperm DNA damage are unknown, it is clear that infertile men possess substantially higher levels of sperm DNA damage than do fertile men. Written by leading, internationally renowned clinicians and basic scientists with expertise in sperm DNA, Sperm Chromatin: Biological and Clinical Applications in Male Infertility and Assisted Reproduction provides readers with a thoughtful and comprehensive review of the biological and clinical significance of sperm DNA damage. The work covers the fundamental principles of sperm chromatin architecture and function, the proposed modes of DNA damage and repair, the tests of sperm DNA damage, the clinical aspects of DNA damage and the impact of DNA damage on reproductive outcome. Unlike any other title on the topic, Sperm Chromatin: Biological and Clinical Applications in Male Infertility and Assisted Reproduction is an invaluable addition to the literature and will serve as an indispensable resource for basic scientists with an interest in sperm biology and for urologists, gynecologists, reproductive endocrinologists, and embryologists working in the field of infertility.

I. Human Sperm Chromatin: Structure and Function

1. Sperm Chromatin: An Overview
Rodney Balhorn

2. Spermatogenesis: An Overview 
Rakesh Sharma and Ashok Agarwal

3. Sperm Nucleoproteins
Rafael Oliva and Judit Castillo

4. The Relationship Between Chromatin Structure and DNA Damage in Mammalian Spermatozoa
Kenneth Dominguez, Chris D. R. Arca, and W. Steven Ward

5. Chromosome Positioning in Spermatozoa
Andrei Zalensky, Olga Mudrak, and Irina Zalenskaya

6. Sperm Mitochondrial DNA
Justin C. St. John and Bianca St. John

7. The Sperm Epigenome
Donovan Chan and Jacquetta Trasler

8. RNA Expression in Germ Cells During Spermatogenesis (Germ Cell Transcriptome) 
Tin-Lap Lee, Albert Hoi-Hung Cheung, Owen M. Rennert, and Wai-Yee Chan

II. Laboratory Evaluation of Sperm Chromatin

9. Sperm Chromatin Structure Assay (SCSA®): 30 Years’ Experience with the SCSA®
Donald P. Evenson

10. Sperm Chromatin Dispersion Test: Technical Aspects and Clinical Applications
Jaime Gosálvez, Carmen López-Fernández, and José Luís Fernández

11. Basic and Clinical Aspects of the Sperm Chromomycin A3 Assay
Giancarlo Manicardi, Davide Bizzaro, and Denny Sakkas

12. Cytochemical Tests for Sperm Chromatin Maturity
Igor Tsarev and Juris Erenpreiss

13. Acridine Orange Test for Assessment of Human Sperm DNA Integrity
Alex C. Varghese, C. Fischer–Hammadeh, and M. E. Hammadeh

14. Laboratory Evaluation of Sperm Chromatin: TUNEL Assay
Rakesh Sharma and Ashok Agarwal

15. The Basic and Clinical Aspects of the Sperm Comet Assay
Luke Simon and Sheena E. M. Lewis

16. Assays Used in the Study of Sperm Nuclear Proteins
Timothy G. Jenkins, Benjamin R. Emery, and Douglas T. Carrell

17. Sperm Epigenetic Profile
Cristina Joana Marques, Alberto Barros, and Mário Sousa

18. Clinical Significance of Sperm RNA
Jean-Pierre Dadoune, Isabelle Galeraud-Denis, and Serge Carreau

III. Biological and Clinical Determinants of Sperm Chromatin Damage

19. Role of Oxidative Stress in the Etiology of Sperm DNA Damage
R. John Aitken and Geoffry N. De Iuliis

20. Abortive Apoptosis and Sperm Chromatin Damage
Hasan M. El-Fakahany and Denny Sakkas

21. Spermiogenesis in Sperm Genetic Integrity
Marie-Chantal Grégoire, Frédéric Leduc, and Guylain Boissonneault

22. Male Subfertility and Sperm Chromatin Damage
Mona Bungum, Aleksander Giwercman, and Marcello Spanò

23. Aging and Sperm DNA Damage
Fábio F. Pasqualotto and Eleonora B. Pasqualotto

24. Cancer in Males: Implications for Sperm Quality, Fertility, and Progeny Outcome
Peter Chan and Bernard Robaire

25. Sperm Chromatin and Environmental Factors
Aleksander Giwercman

26. Effects of Male Accessory Gland Infection on Sperm Parameters
Aldo E. Calogero, Sandro La Vignera, Rosita A. Condorelli, Rosario D’Agata, and Enzo Vicari

IV. Sperm Chromatin and Assisted Reproductive Technology Outcomes

27. The Impact of Sperm Processing and Cryopreservation on Sperm DNA Integrity
Dan Yu, Luke Simon, and Sheena E. M. Lewis

28. Structure of Chromatin in Human Sperm Bound to Hyaluronic Acid: The Benefits of PICSI Dish Mediated Sperm Selection
Leyla Sati and Gabor Huszar

29. Electrophoretic Sperm Separation
Steven Fleming and R. John Aitken

30. Antioxidants and Sperm DNA Damage
Armand Zini and Maria San Gabriel

31. Sperm Chromatin and ART (IUI, IVF, and ICSI) Pregnancy
Mona Bungum

32. Sperm DNA Damage and Pregnancy Loss After IVF/ICSI
Armand Zini and Jason Matthew Boman

33. Postnatal Effects of Sperm Chromatin Damage
Miriam Pérez-Crespo, Raúl Fernández-González, Miguel Ángel Ramírez, Eva Pericuesta, Alexandra Calle, and Alfonso Gutiérrez-Adán

34. Evaluation of Chromatin and DNA Integrity in Testicular Sperm
Armand Zini and Naif Al-Hathal

V. Protocols and Integrity Tests

35. The Alkaline Comet Assay
Sheena E. M. Lewis

36. Sperm Chromatin Structure Assay (SCSA)
Donald P. Evenson

37. Measurement of DNA Damage in Spermatozoa by TUNEL Assay
Rakesh Sharma and Ashok Agarwal

38. Clinical Utility of Sperm DNA Integrity Tests
Armand Zini