Practical Manual of In Vitro Fertilization

This practical volume provides a complete review of the most well-established and current diagnostic and treatment techniques comprising in vitro fertilization. Each chapter includes a brief abstract, a keyword glossary, a step-by-step protocol of the laboratory procedures, and expert commentary.

The Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is a unique, accessible title that provides a complete review of the most well-established and current diagnostic and treatment techniques comprising in vitro fertilization. Throughout the chapters, a uniform structure is employed, including a brief abstract, a keyword glossary, a step-by-step protocol of the laboratory procedures, several pages of expert commentary, key issues of clinical concern, and a list of references. The result is a readily accessible, high quality reference guide for reproductive endocrinologists, urologists, embryologists, biologists and research scientists. The Manual also offers an excellent description of novel procedures that will likely be employed in the near future.  An indispensable resource for physicians and basic scientists, the Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is an invaluable reference andaddition to the literature.

Journey of Human Gametes In Vitro: 1978 to 2010.- Building the Laboratory.- Air Quality Management.- Organizational Aspects of the Laboratory in a Tertiary Care ART Center.- Quality Control Management.- Daily, Weekly, and Regular Preparations for the IVF Laboratory.- Risk and Safety in the IVF Clinic.- Essential Instruments and Disposable Supplies for an IVF Laboratory.- Co2 and Low-O2 Incubators.- IVF Workstations.- Culture Media in IVF: Decisions for the Laboratory.- Oocyte Denuding.- Assessment of Oocyte Quality.- Polarization Microscopy.- Cumulus Cell Gene Expression in Assessment of Oocyte Quality.- Short Culture: Day 1/Day 2/Day 3 Embryo Culture.- Extended Culture in IVF.- In Vitro Maturation of Human Oocytes.- In Vivo Embryo Culture Device.- Microfluidics for Gamete Manipulation and Embryo Culture.- Sperm Assessment: Traditional Approaches and Their Indicative Value.- Sperm Assessment: Novel Approaches and Their Indicative Value.- Sperm Processing for IVF.- PESA/TESA/TESE Sperm Processing.- Processing Sperm Samples in HIV-Positive Patients.- Intracytoplasmic Morphologically Selected Sperm Injection.- Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in the Andrology and IVF Laboratories.-  Electrophoretic Sperm Separation.- Magnetic Activated Cell Sorting of Human Spermatozoa.- Poloscope-Based Sperm Selection.- Intrauterine Insemination.- Conventional IVF Insemination.- Intracytoplasmic Sperm Injection.- Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization.- Hydraulic Manipulators for ICSI.- Research Instruments Micromanipulators.- Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators.- The Leica-IMSI System.- Automated Robotic Intracytoplasmic Sperm Injection.- Oocyte Treatment and Preparation for Microinjection.- Livestock Production Via Micromanipulation.- Pronuclear Scoring in Human In Vitro Fertilization.- Cumulative Morphological Assessment of Embryo Quality.- Metabolomics: The ViaMetrics-E™ Procedure for Assessing Embryo Viability.- Oxygen Consumption as an Indicator of Oocyte and Embryo Viability.- Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection.- Amino Acid Turnover as a Biomarker of Embryo Viability.- Real-time Embryo Monitoring Devices for Embryo Selection.- Assisted Hatching in IVF.- Polar Body Biopsy.- Cleavage Stage Embryo Biopsy.- Embryo Biopsy for PGD: Current Perspective.- Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects.- Sperm Cryopreservation.- Slow Freezing of Oocytes.- Vitrification: Research in Animal Models.- Oocyte Vitrification.- Slow Freezing of Embryos.- Vitrification of Embryos.- Directional Freezing of Reproduction Cells, Tissues, and Organs.- Ovarian Tissue Cryopreservation.- Assessment of Uterine Receptivity.- Single Embryo Transfer.- Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based Evaluation.- Cumulus-Aided Embryo Transfer.- Data Management in the ART Laboratory: Requirements and Solutions.- Regulation, Licensing, and Accreditation of the ART Laboratory.- Legislation in the United Kingdom.- Regulation, Licensing, and Accreditation of the ART Laboratory in Europe.- Regulation, Licensing, and Accreditation of ART Laboratories in India.- Troubleshooting in the Clinical Embryology Laboratory: The Art of Problem-Solving in ART.- The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development.- Nuclear and Cytoplasmic Transfer: Human Applications and Concerns.- Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization.- Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence.