Chemistry of Metalloproteins

JOSEPH J. STEPHANOS, PHD, is Associate Professor of Bioinorganic, Biophysical, and Inorganic Chemistry at Menofia University. His research examines metalloprotein chemistry, ligand-binding, and coordination chemistry of biologically active compounds.
 
ANTHONY W. ADDISON, PHD, is Professor of Inorganic Chemistry at Drexel University. His research focuses on bioinorganic chemistry; chemistry of dioxygen- and NO-binding metalloproteins; and the design, synthesis, and properties of novel chelating agents and macrocyclic and oligonuclear metal complexes. His numerous research articles and conference presentations have earned over 8,000 citations and a Hirsch Index of 37.

Addresses the full gamut of questions in metalloprotein science
 
Formatted as a question-and-answer guide, this book examines all major families of metal binding proteins, presenting our most current understanding of their structural, physicochemical, and functional properties. Moreover, it introduces new and emerging medical applications of metalloproteins. Readers will discover both the underlying chemistry and biology of this important area of research in bioinorganic chemistry.
 
Chemistry of Metalloproteins features a building block approach that enables readers to master the basics and then advance to more sophisticated topics. The book begins with a general introduction to bioinorganic chemistry and metalloproteins. Next, it covers:
* Alkali and alkaline earth cations
* Metalloenzymes
* Copper proteins
* Iron proteins
* Vitamin B12
* Chlorophyll
 
Chapters are richly illustrated to help readers fully grasp all the chemical concepts that govern the biological action of metalloproteins. In addition, each chapter ends with a list of suggested original research articles and reviews for further investigation of individual topics.
 
Presenting our most current understanding of metalloproteins, Chemistry of Metalloproteins is recommended for students and researchers in coordination chemistry, biology, and medicine.
 
Each volume of the Wiley Series in Protein and Peptide Science addresses a specific facet of the field, reviewing the latest findings and presenting a broad range of perspectives. The volumes in this series constitute essential reading for biochemists, biophysicists, molecular biologists, geneticists, cell biologists, and physiologists as well as researchers in drug design and development, proteomics, and molecular medicine with an interest in proteins and peptides.

Preface ix
 
1 Introduction 1
 
Proteins: Formation, Structures, and Metalloproteins, 4
 
References, 28
 
2 Alkali and Alkaline Earth Cations 31
 
References, 67
 
3 Nonredox Metalloenzymes 71
 
Carboxypeptidases, 75
 
Carbonic Anhydrase, 84
 
Alcohol Dehydrogenase, 88
 
References, 91
 
4 Copper Proteins 95
 
Introduction, 95
 
Electronic Spectra of Copper Ions, 96
 
ESR Spectra of Copper Ions, 105
 
Copper Proteins, 117
 
Plastocyanin, 119
 
Azurin and Stellacyanin, 127
 
Superoxide Dismutase, 131
 
Hemocyanin, 135
 
Ascorbic Oxidase, 139
 
References, 142
 
5 Iron Proteins 147
 
Introduction, 147
 
Electronic Spectra of Iron Ions, 148
 
Mössbauer Spectroscopy of Iron Ions, 155
 
ESR Spectra of Iron (III), 161
 
Iron Bioavailability, 166
 
Siderophores, 171
 
Iron Storage and Transfer Proteins, 184
 
Ferritin, 184
 
Transferrin, 187
 
Dioxygenase Iron Proteins, 195
 
Iron-Sulfur Proteins, 207
 
Rubredoxin, 207
 
Ferredoxins, 212
 
2Fe-2S Ferredoxins, 212
 
4Fe-4S Ferredoxins, 221
 
Aconitase, 226
 
Hydroxylases, 229
 
Hydrogenases, 232
 
Nitrogenases, 240
 
Binuclear Iron Proteins, 251
 
Hemerythrin, 253
 
Ribotide Reductase, Purple Acid Phosphate, and Methane Monooxygenase, 260
 
Hemoproteins: Classification and Behavior of Heme in Absence of Globins, 267
 
Myoglobin and Hemoglobin, 275
 
Myoglobin, 275
 
Hemoglobin, 280
 
Cytochrome C, 298
 
Electron Transfer in Porphyrins and Metalloporphyrins, 301
 
Catalases, 311
 
Peroxidases, 315
 
Cytochrome P-450, 322
 
Electronic Spectra of Hemoproteins, 327
 
ESR Spectra of Hemoproteins, 362
 
References, 378
 
6 Vitamin B12 393
 
References, 405
 
7 Chlorophyll 407
 
References, 421
 
Index 423