Intelligent Surfaces in Biotechnology

A comprehensive overview of smart and responsive surfaces in biotechnology and their applications A wave of recent advances in cell biology, biophysics, chemistry, and materials science has enabled the development of a new generation of smart biomaterials. Intelligent Surfaces in Biotechnology: Scientific and Engineering Concepts, Enabling Technologies, and Translation to Bio-Oriented Applications provides readers with a comprehensive overview of surface modifications and their applications, including coverage of the physico-chemical properties, characterization methods, smart coating technologies, and demonstration of performance in vitro and in vivo. The first part of the book covers applications in the fields of biosensing and biodiagnostics, while the second part focuses more on coatings for medical devices, drug delivery, and tailored cell-surface interactions. The book explores intelligent surface applications such as tissue engineering, drug targeting and delivery, wound healing and anti-infection strategies, biosensors, nanopatterning, and bioinspired design of novel responsive materials and multifunctional surfaces. Designed to aid scientists and engineers in understanding the rapidly developing field of biofunctional surfaces, Intelligent Surfaces in Biotechnology is an edited volume with each chapter written by a respected expert and featuring examples taken from the most state-of-the-art developments in the discipline. Cover Image: Design concept for a diagnostic microfluidic system based on responsive polymer- and antibody-conjugated nanobeads (see Chapter 2 of this book, Figure 2.5; reproduced by permission from the Royal Society of Chemistry).

A comprehensive overview of smart and responsive surfaces inbiotechnology and their applicationsA wave of recent advances in cell biology, biophysicschemistry, and materials science has enabled the development of anew generation of smart biomaterials. Intelligent Surfaces inBiotechnology: Scientific and Engineering Concepts, EnablingTechnologies, and Translation to Bio-Oriented Applications providesreaders with a comprehensive overview of surface modifications andtheir applications, including coverage of the physico-chemicalproperties, characterization methods, smart coating technologiesand demonstration of performance in vitro and in vivo.The first part of the book covers applications in the fields ofbiosensing and biodiagnostics, while the second part focuses moreon coatings for medical devices, drug delivery, and tailoredcell-surface interactions. The book explores intelligent surfaceapplications such as tissue engineering, drug targeting anddelivery, wound healing and anti-infection strategies, biosensorsnanopatterning, and bioinspired design of novel responsivematerials and multifunctional surfaces.Designed to aid scientists and engineers in understanding therapidly developing field of biofunctional surfaces, IntelligentSurfaces in Biotechnology is an edited volume with each chapterwritten by a respected expert and featuring examples taken from themost state-of-the-art developments in the discipline.Cover Image: Design concept for a diagnostic microfluidic systembased on responsive polymer- and antibody-conjugated nanobeads (seeChapter 2 of this book, Figure 2.5; reproduced by permission fromthe Royal Society of Chemistry).

Chapter 1. Responsive Polymers as Intelligent Coatings for Biosensors: Architectures, Response Mechanisms, and ApplicationsVinalia Tjong, Jianming Zhang, Ashutosh Chilkoti and Stefan Zauscher1.1 Introduction1.2 SRP Architectures for Biosensor Applications1.3 Mechanisms of Response1.4 Sensing and Transduction Mechanisms1.5 Limitations and Challenges1.6 Conclusion and OutlookChapter 2. Smart Surfaces for Point-of-Care DiagnosticsMichael A. Nash, Allison L. Golden, John M. Hoffman, James Lai, and Patrick S. Stayton2.1 Introduction2.2 Standard Methods for Biomarker Purification, Enrichment, and Detection2.3 Smart Reagents for Biomarker Purification and Processing2.4 Sample-Processing Modules for Smart Conjugate Bioassays2.5 Devices for use in Smart Conjugate Bioassays2.6 ConclusionsChapter 3. Design of Intelligent Surface Modifications and Optimal Liquid Handling for Nanoscale Bioanalytical SensorsLaurent Feuz, Fredrik Höök and Erik Reimhult3.1 Introduction3.2 Orthogonal small (nano) scale surface modification using molecular self-assembly3.3 Alternative surface patterning strategies3.4 The challenge of analytic transport3.5 Concluding remarksChapter 4. Intelligent Surfaces for Effect Based Nano-biosensingAkira Matsumoto, Yuji Miyahara and Kazunori Kataoka4.1 Introduction4.2 Field effect transistor based biosensors4.3 Intelligent surfaces for signal transduction and amplification of Bio-FETs4.4 New targets of Bio-FETs4.5 Future PerspectiveChapter 5. Supported Lipid Bilayers: Intelligent Surfaces for Ion Channel RecordingsAndreas Janshoff and Claudia Steinem5.1 Introduction5.2 Supported lipid bilayers5.3 Characterizatics of SSMs5.4 Ion channels in SSMs5.5 Future perspective: Ion channels in micropatterned membranesChapter 6. Antimicrobial and Anti-inflammatory Intelligent SurfacesHans J. Griesser, Heike Hall, Toby Jenkins, Stefani S. Griesser and Krasimir Vasilev6.1 Introduction6.2 Antibacterial strategies6.3 Bioactive antibacterial surfaces6.4 Stimulus-responsive antibacterial coatings for wound dressings6.5 Anti-inflammatory surfaces6.6 Conclusions and OutlookChapter 7. Intelligent Polymer Thin Films and Coatings for Drug DeliveryAlexander N. Zelikin and Brigitte Städler7.1 Introduction7.2 Surface Mediated Drug Delivery7.3 Drug Delivery Vehicles With Functional Polymer Coatings7.4 OutlookChapter 8. Micro- and Nanopatterning of Active Biomolecules and CellsDaniel Aydin, Vera C. Hirschfeld-Warneken, Ilia Louban and Joachim P. Spatz8.1 Introduction8.2 Chemical Approaches for Protein Immobilization8.3 Biomolecule patterning by "top-down" techniques8.4 Biomolecule Nanoarrays bu Block Copolymer Nanolithography8.5 Application of Nanostructured Surfaces to Study Cell Adhesion8.6 ConclusionChapter 9. Responsive Polymer Coatings for Smart Applications in Cromatography, Drug Delivery Systems and Cell Sheet EngineeringRogério P. Pirraco, Masayuki Yamato, Yoshikatsu Akiyama, Kenichi Nagase, Masamichi Nakayama, Alexandra P. Marques, Rui L. Reis and Teruo Okano9.1 Introduction9.2 Temperature-responsive chromatography9.3 Temperature-responsive polymer micelle9.4 Temperature-responsive culture surfaces9.5 Cell sheet Engineering9.6 Conclusions