Protein Purification

Dr Philip Bonner is Programme Leader for the MRes in Applied Biosciences at Nottingham Trent University.

The second edition of Protein Purification expands on the topics covered previously. It discusses the structure and properties of water and emphasizes properties which can be exploited to aid purification. It features an updated suppliers list and chapters feature an extension of protocols that are discussed.

1. Water, pH and buffers. 2. Protein structure and properties. 3. Chromatography and the strategy of protein purification. 4. Groundwork. 5. Non-affinity absorption techniques used to purify proteins. 6. Affinity-based procedures used to purify proteins. 7. Non-absorption techniques for purifying proteins. 8. Methods for monitoring the purity of protein solutions. Appendix 1: The laws of thermodynamics and the Gibbs free energy equation. Appendix 2: Properties of amino acids. Appendix 3: The common units used in biology. Appendix 4: Chromatographic runs. Appendix 5: Determining the concentration of a compound in a solution in parts per million (ppm). Appendix 6: The answers to the exercises in the various chapters. Appendix 7: An alphabetical list of chromatography, electrophoresis, filtration equipment and laboratory suppliers. Appendix 8: Buffer tables to prepare at a required pH value.