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Single-particle Cryo-EM of Biological Macromolecules
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Artikel-Nr:
9780750330374
Veröffentl:
2021
Erscheinungsdatum:
19.05.2021
Seiten:
120
Autor:
Robert M Glaeser
Gewicht:
754 g
Format:
261x184x23 mm
Sprache:
Englisch
Beschreibung:
Professor Glaeser (UC Berkeley) made major contributions to the initial development of cryo-EM technology, and he currently is active in the improvement of specimen preparation.
This edited book is written for students, postdocs and established investigators who want to enter the field of single-particle cryo-EM. This is a recently developed method to determine high-resolution structures of biological macromolecules. A major strength is the fact that cryo-EM does not require prior crystallization of protein complexes. It is especially well suited for larger complexes and molecular machines. This book, provides a comprehensive, accessible and authoritative introduction to the field. It covers all necessary background, ranging from the underlying concepts to practical aspects such as specimen preparation, data-collection, data analysis, and the final validation of results.