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Autor: Gordon G. Hammes
ISBN-13: 9780471733546
Einband: E-Book
Seiten: 184
Sprache: Englisch
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Spectroscopy for the Biological Sciences

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An introduction to the physical principles of spectroscopy andtheir applications to the biological sciences
Advances in such fields as proteomics and genomics place newdemands on students and professionals to be able to applyquantitative concepts to the biological phenomena that they arestudying. Spectroscopy for the Biological Sciences providesstudents and professionals with a working knowledge of the physicalchemical aspects of spectroscopy, along with their applications toimportant biological problems.

Designed as a companion to Professor Hammes's Thermodynamics andKinetics for the Biological Sciences, this approachable yetthorough text covers the basic principles of spectroscopy,including:
* Fundamentals of spectroscopy
* Electronic spectra
* Circular dichroism and optical rotary dispersion
* Vibration in macromolecules (IR, Raman, etc.)
* Magnetic resonance
* X-ray crystallography
* Mass spectrometry

With a minimum of mathematics and a strong focus on applications tobiology, this book will prepare current and future professionals tobetter understand the quantitative interpretation of biologicalphenomena and to utilize these tools in their work.
PREFACE.

1. FUNDAMENTALS OF SPECTROSCOPY.

Introduction.

Quantum Mechanics.

Particle in a Box.

Properties of Waves.

References.

Problems.

2. X-RAY CRYSTALLOGRAPHY.

Introduction.

Scattering of X Rays by a Crystal.

Structure Determination.

Neutron Diffraction.

Nucleic Acid Structure.

Protein Structure.

Enzyme Catalysis.

References.

Problems.

3. ELECTRONIC SPECTRA.

Introduction.

Absorption Spectra.

Ultraviolet Spectra of Proteins.

Nucleic Acid Spectra.

Prosthetic Groups.

Difference Spectroscopy.

X-Ray Absorption Spectroscopy.

Fluorescence and Phosphorescence.

RecBCD: Helicase Activity Monitored by Fluorescence.

Fluorescence Energy Transfer: A Molecular Ruler.

Application of Energy Transfer to Biological Systems.

Dihydrofolate Reductase .

References .

Problems.

4. CIRCULAR DICHROISM, OPTICAL ROTARY DISPERSION, ANDFLUORESCENCE POLARIZATION.

Introduction.

Optical Rotary Dispersion.

Circular Dichroism.

Optical Rotary Dispersion and Circular Dichroism ofProteins.

Optical Rotation and Circular Dichroism of Nucleic Acids.

Small Molecule Binding to DNA.

Protein Folding.

Interaction of DNA with Zinc Finger Proteins.

Fluorescence Polarization.

Integration of HIV Genome into Host Genome.

?-Ketoglutarate Dehyrogenase.

References.

Problems.

5. VIBRATIONS IN MACROMOLECULES.

Introduction.

Infrared Spectroscopy.

Raman Spectroscopy.

Structure Determination with Vibrational Spectroscopy ResonanceRaman Spectroscopy.

Structure of Enzyme-Substrate Complexes.

References.

Problems.

6. PRINCIPLES OF NUCLEAR MAGNETIC RESONANCE AND ELECTRON SPINRESONANCE.

Introduction.

NMR Spectrometers.

Chemical Shifts.

Spin-Spin Splitting.

Relaxation Times.

Multidimensional NMR.

Magnetic Resonance Imaging.

Electron Spin Resonance.

References.

Problems.

7. APPLICATIONS OF MAGNETIC RESONANCE TO BIOLOGY.

Introduction.

Regulation of DNA Transcription.

Protein-DNA Interactions.

Dynamics of Protein Folding.

RNA Folding.

Lactose Permease.

Conclusion.

References.

8. MASS SPECTROMETRY.

Introduction.

Mass Analysis.

Tandem Mass Spectrometry (MS/MS)..

Ion Detectors.

Ionization of the Sample.

Sample Preparation/Analysis.

Proteins and Peptides.

Protein Folding.

Other Biomolecules.

References.

Problems.

APPENDICES.

1. Useful Constants and Conversion Factors.

2. Structures of the Common Amino Acids at Neutral pH.

3. Common Nucleic Acid Components.

INDEX.

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Autor: Gordon G. Hammes
ISBN-13 :: 9780471733546
ISBN: 0471733547
Verlag: John Wiley & Sons
Seiten: 184
Sprache: Englisch
Auflage 1. Auflage
Sonstiges: Ebook