Transmembrane Transporters

A must-have far-reaching text that provides readers with a state-of-the-art molecule update on transmembrane transporters, focusing on the methodological approaches currently employed to better understand how transporters work and how they can be used in cutting edge therapies. Each chapter begins with an overview of the importnat biological questions presently being considered in their field, then presents scientific approaches to address these questions. In explaining approaches, the authors cover bench-top protocols, conceptual frameworks, data obtained, and pitfalls common to the techniques.

A must-have far-reaching text that provides readers with a state-of-the-art molecule update on transmembrane transporters, focusing on the methodological approaches currently employed to better understand how transporters work and how they can be used in cutting edge therapies. Each chapter begins with an overview of the importnat biological questions presently being considered in their field, then presents scientific approaches to address these questions. In explaining approaches, the authors cover bench-top protocols, conceptual frameworks, data obtained, and pitfalls common to the techniques.

Series Preface.Preface.Contributors.Families of Transporters and Their Classification (M. Saier).Yeast as a Model System for Studying Glucose Transport (E.Boles).Neurotransmitter Transporters of Drosophila (M. Burg & W.Pak).Transgenic Mice in Monoamine Transporter Research (S. Jones).Searching for Novel Genetic Variation in NeurotransmitterTransporters (R. Blakely & A. Belous).Nonviral Gene Transfer Allows Up- and Down-Expression of the BrainSerotonin Transporter with Functional Consequences (M. Martres, etal.).Methods in Studying the Regulation and Trafficking of TransmembraneTransporters (S. Deken, et al.).Chemical Modification Strategies for Structure-Function Studies (G.Rudnick).Methanethiosulfonate Reagent Accessibility StudiesCysteine-Scanning Mutagenesis, Protein Overexpression, andFunctional Reconstitution: A Strategy for Studying theStructure/Function Relationships within the Mitochondrial CitrateTransport Protein (R. Kaplan).Peptide Mapping of Dopamine Transporter Ligand and SubstrateInteraction Sites (M. Lowe, et al.).Mass Spectrometry of Membrane Transport Proteins (J. Whitelegge, etal.).Amperometric Recording of Amphetamine-Induced Dopamine Efflux (D.Sulzer, et al.).Voltage Clamp and Fluorometric Techniques for Studying GlutamateTransporter Function (A. Tzingounis, et al.).Studies of Glial Glutamate Transporters in HippocampalMicrocultures (S. Mennerick, et al.).Imaging Monoamine Transporters in the Brain (H. Kung & M.Kung).Measuring and Modeling the Spatiotemporal Profile of GABA at theSynapse (L. Overstreet, et al.).Index.