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Autor: Philip Bonner
ISBN-13: 9780470972052
Einband: E-Book
Seiten: 232
Sprache: Englisch
eBook Typ: PDF
eBook Format: E-Book
Kopierschutz: Adobe DRM [Hard-DRM]
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Basic Bioscience Laboratory Techniques

A Pocket Guide
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This unique, practical, pocket-sized guide and reference providesevery first year bioscience student with all they need to know toprepare reagents correctly and perform fundamental laboratorytechniques. It also helps them to analyse their data and presenttheir findings, in addition to directing the reader, via acomprehensive list of references, to relevant further reading
All of the core bioscience laboratory techniques are coveredincluding: basic calculations and the preparation of solutions;aseptic techniques; microscopy techniques; cell fractionation ;spectrophotometry; chromatography of small and large molecules:electrophoresis of proteins and nucleic acids and data analysis. Inaddition the book includes clear, relevant diagrams and workedexamples of calculations.

In short, this is a 'must-have' for all first year biosciencestudents struggling to get to grips with this vitally importantelement of their course.
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Preface ix

Glossary xi

Abbreviations xvii

1 The preparation of solutions in bioscience research1

1.1 Introduction 1

1.2 Concentration 2

1.3 Using balances to weigh out reagents 3

1.4 Practical considerations when making a 1.0 M solution 6

1.5 Dilutions and the use of pipettes 11

1.6 Water, acids and bases 15

1.7 Buffers 21

1.8 The equilibrium/dissociation constant (Ka) for an acid orbase and the Henderson-Hasselbalch equation 24

1.9 Summary 26

2 Microscopy 27

2.1 Introduction 27

2.2 Microscopes - general principles 29

2.3 Principles of image formation 30

2.4 Light microscopy 32

2.5 Electron microscopy 46

2.6 Summary 49

3 Spectrophotometry 51

3.1 Introduction 51

3.2 The electromagnetic spectrum 51

3.3 The absorbance of light 54

3.4 Absorbtion spectrophotometry 56

3.5 The laws of the absorbance of light 57

3.6 The Beer Lambert law 61

3.7 Spectrophotometers 63

3.8 Applications of spectrophotometry in bioscience 68

3.9 Summary 73

4 Data analysis and presentation 74

4.1 Introduction 74

4.2 Statistical analysis of data: some key definitions 76

4.3 Distributions 79

4.4 Statistical comparison of data 85

4.5 Presentation, structure and organization of data inlaboratory reports 94

4.6 Summary 100

5 The extraction and clarification of biological material103

5.1 General introduction 103

5.2 Extraction 103

5.3 Extraction methods for animal and plant tissue 105

5.4 Extraction methods for bacteria 107

5.5 Clarification 108

5.6 Centrifugation techniques 115

5.7 Points of good practice in centrifugation 119

5.8 Summary 120

6 Electrophoresis of proteins and nucleic acids 121

6.1 General introduction 121

6.2 Separation of protein mixtures by gel electrophoresis122

6.3 Other electrophoretic techniques applied to proteins 136

6.4 Separation of nucleic acids by gel electrophoresis 137

6.5 Applications of gel electrophoresis of nucleic acids 141

6.6 Summary 146

7 Chromatography 147

7.1 General introduction 147

7.2 The theory of chromatography 147

7.3 Factors to consider in chromatography 152

7.4 Methods used to elute samples in chromatography 153

7.5 Different types of chromatography and what properties can beused to separate molecules 153

7.6 Thin layer chromatography (TLC) 156

7.7 High pressure liquid chromatography (HPLC) 158

7.8 Gas liquid chromatography (GLC) 161

7.9 Ion exchange chromatography (IEX) 162

7.10 Size exclusion chromatography 164

7.11 Affinity chromatography 166

7.12 Summary 167

8 Cell culture techniques 169

8.1 Introduction 169

8.2 Growth and maintenance of cells in culture 174

8.3 Summary 192

Suggestions for further reading 195

Index 198

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Autor: Philip Bonner
ISBN-13 :: 9780470972052
ISBN: 047097205X
Verlag: John Wiley & Sons
Seiten: 232
Sprache: Englisch
Auflage 1. Auflage
Sonstiges: Ebook