Advanced Techniques in Diagnostic Cellular Pathology

In recent years cellular pathology has become more closely involved in the direct management of patients with the introduction of molecular technologies and targeted therapies. Advanced Techniques in Diagnostic Cellular Pathology introduces students and professionals to these concepts and the key technologies that are influencing clinical practice today. Each chapter is carefully structured to introduce the very latest techniques and describe their clinical purpose, principle, method and application in cellular pathology. The advantages of various methods for preparing, observing and demonstrating cells and tissues employed to assist in diagnosis are explored, in addition to the use of quantitative methods in the detection and diagnosis of disease. Supplementary web-based material including annotated virtual microscope slides is available with the book. This is provided courtesy of i-Path Diagnostics Ltd and can be accessed online from their website pathxl.com Describes the very latest, emerging and established molecular aspects of diagnostic pathology. A clear, focused approach with each chapter containing a summary, a review of basic principles and clinical applications. Includes web-based annotated virtual microscope slides. Contributions from experienced practitioners contain numerous real-world examples illustrating the use of different diagnostic techniques, and their clinical relevance Written by a team of experienced practitioners this book will prove invaluable both to postgraduate biomedical science students who are training to be cellular pathologists and to professionals working in diagnostic and research laboratories as part of their continuing professional development.

In recent years cellular pathology has become more closely involvedin the direct management of patients with the introduction ofmolecular technologies and targeted therapies. AdvancedTechniques in Diagnostic Cellular Pathology introduces studentsand professionals to these concepts and the key technologies thatare influencing clinical practice today.Each chapter is carefully structured to introduce the verylatest techniques and describe their clinical purpose, principlemethod and application in cellular pathology. The advantages ofvarious methods for preparing, observing and demonstrating cellsand tissues employed to assist in diagnosis are explored, inaddition to the use of quantitative methods in the detection anddiagnosis of disease. Supplementary web-based material includingannotated virtual microscope slides is available with the book.This is provided courtesy of i-Path Diagnostics Ltd and can beaccessed online from their website pathxl.com* Describes the very latest, emerging and established molecularaspects of diagnostic pathology.* A clear, focused approach with each chapter containing asummary, a review of basic principles and clinicalapplications.* Includes web-based annotated virtual microscope slides.* Contributions from experienced practitioners contain numerousreal-world examples illustrating the use of different diagnostictechniques, and their clinical relevanceWritten by a team of experienced practitioners this book willprove invaluable both to postgraduate biomedical science studentswho are training to be cellular pathologists and to professionalsworking in diagnostic and research laboratories as part of theircontinuing professional development.

List of Contributors viiPreface ixList of Tables xiList of Figures xiiiSupplementary Materials xvii1 Virtual Microscopy 1Jim Diamond and David McCleary1.1 Introduction 11.2 Digital (virtual) microscopy: equipment for implementation 21.3 The virtual slide format 111.4 Image serving and viewing 131.5 Applications of virtual microscopy 141.6 Virtual microscopy in education 231.7 Computational aspects of virtual microscopy 301.8 Conclusions 352 Cytopathology 37Mary Hannon-Fletcher2.1 Introduction 372.2 Basic principles 382.3 Cytodiagnosis 442.4 Gynaecological cytopathology 492.5 Conclusions 643 Flow Cytometry 69Ian Dimmick3.1 Introduction 693.2 Sample preparation 723.3 Principles of the flow cytometer 733.4 Clinical applications 833.5 The future for flow cytometry 953.6 Conclusions 974 Immunocytochemistry 99Perry Maxwell and Merdol Ibrahim4.1 Introduction 994.2 Basic principles 1004.3 Clinical immunocytochemistry 1104.4 Conclusions 1305 Microarray-based Comparative Genomic Hybridization 135David S.P. Tan, Rachael Natrajan and Jorge S. Reis-Filho5.1 Introduction 1355.2 Principles of array CGH 1385.3 aCGH platforms 1405.4 Choosing the right platform 1445.5 Analysis and validation 1465.6 Finding the target 1475.7 Data integration 1525.8 Clinical applications 1525.9 Conclusions 1536 Tissue In Situ Hybridization 163Anthony O' Grady, John O' Loughlin and Hilary Magee6.1 Introduction 1636.2 ISH probes 1646.3 Probe labels 1666.4 ISH detection systems 1686.5 Tissue preparation and the ISH procedure 1686.6 Signal amplification 1706.7 ISH controls 1716.8 Clinical applications of tissue ISH 1726.9 ISH automation 1796.10 Image capture and analysis 1806.11 Recent developments and future directions in tissue ISH 1816.12 Conclusions 183Index 189