Molecular Genetics of Bacteria

The fifth edition of this highly successful book provides students with an essential introduction to the molecular genetics of bacteria covering the basic concepts and the latest developments. It is comprehensive, easy to use and well structured with clear two-colour diagrams throughout.
 
Specific changes to the new edition include:
* More detail on sigma factors, anti-sigma factors and anti-anti sigma factors, and the difference in the frequency of sigma factors in bacteria
* Expand material on integrons as these are becoming increasingly important in antibiotic resistance
* Enhanced treatment of molecular phylogeny
* Complete revision and updating of the final chapter on 'Gene Mapping and Genomics'
* Two-colour illustrations throughout.
 

The focus of the book remains firmly on bacteria and will be invaluable to students studying microbiology, biotechnology, molecular biology, biochemistry, genetics and related biomedical sciences.

Preface.
 
1 Nucleic Acid Structure and Function.
 
1.1 Structure of nucleic acids.
 
1.1.1 DNA.
 
1.1.2 RNA.
 
1.1.3 Hydrophobic interactions.
 
1.1.4 Different forms of the double helix.
 
1.1.5 Supercoiling.
 
1.1.6 Denaturation and hybridization.
 
1.1.7 Orientation of nucleic acid strands.
 
1.2 Replication of DNA.
 
1.2.1 Unwinding and rewinding.
 
1.2.2 Fidelity of replication; proofreading.
 
1.3 Chromosome replication and cell division.
 
1.4 DNA repair.
 
1.4.1 Mismatch repair.
 
1.4.2 Excision repair.
 
1.4.3 Recombination (post-replication) repair.
 
1.4.4 SOS repair.
 
1.5 Gene expression.
 
1.5.1 Transcription.
 
1.5.2 Translation.
 
1.5.3 Post-translational events.
 
1.6 Gene organization.
 
2 Mutation and Variation.
 
2.1 Variation and evolution.
 
2.1.1 Fluctuation test.
 
2.1.2 Replica plating.
 
2.1.3 Directed mutation in bacteria?
 
2.2 Types of mutation.
 
2.2.1 Point mutations.
 
2.2.2 Conditional mutants.
 
2.2.3 Variation due to larger-scale DNA alterations.
 
2.2.4 Extrachromosomal agents and horizontal gene transfer.
 
2.3 Recombination.
 
2.3.1 A model of the general (homologous) recombination process.
 
2.3.2 Enzymes involved in recombination.
 
2.4 Phenotypes.
 
2.4.1 Restoration of phenotype.
 
2.5 Mechanisms of mutation.
 
2.5.1 Spontaneous mutation.
 
2.5.2 Chemical mutagens.
 
2.5.3 Ultraviolet irradiation.
 
2.6 Isolation and identification of mutants.
 
2.6.1 Mutation and selection.
 
2.6.2 Replica plating.
 
2.6.3 Isolation of other mutants.
 
2.6.4 Molecular methods.
 
3 Regulation of Gene Expression.
 
3.1 Gene copy number.
 
3.2 Transcriptional control.
 
3.2.1 Promoters.
 
3.2.2 Terminators, attenuators and anti-terminators.
 
3.2.3 Induction and repression: regulatory proteins.
 
3.2.4 Two-component regulatory systems.
 
3.2.5 Global regulatory systems.
 
3.2.6 Quorum sensing.
 
3.3 Translational control.
 
3.3.1 Ribosome binding.
 
3.3.2 Codon usage.
 
3.3.3 Stringent response.
 
3.3.4 Regulatory RNA.
 
3.4 Phase variation.
 
4 Genetics of Bacteriophages.
 
4.1 Bacteriophage structure.
 
4.2 Single-strand DNA bacteriophages.
 
4.2.1 ÕX174.
 
4.2.2 M13.
 
4.3 RNA-containing phages: MS2.
 
4.4 Double-stranded DNA phages.
 
4.4.1 Bacteriophage T4.
 
4.4.2 Bacteriophage ".
 
4.4.3 Lytic and lysogenic regulation of bacteriophage ".
 
4.5 Restriction and modification.
 
4.6 Bacterial resistance to phage attack.
 
4.7 Complementation and recombination.
 
4.8 Why are bacteriophages important?
 
4.8.1 Phage typing.
 
4.8.2 Phage therapy.
 
4.8.3 Phage display.
 
4.8.4 Phages in the natural environment.
 
4.8.5 Bacterial virulence and phage conversion.
 
5 Plasmids.
 
5.1 Some bacterial characteristics are determined by plasmids.
 
5.1.1 Antibiotic resistance.
 
5.1.2 Colicins and bacteriocins.
 
5.1.3 Virulence determinants.
 
5.1.4 Plasmids in plant-associated bacteria.
 
5.1.5 Metabolic activities.
 
5.2 Molecular properties of plasmids.
 
5.2.1 Plasmid replication and control.
 
5.2.2 Partitioning.

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