Enzyme Technologies

An authoritative review of the latest developments in the chemical biology of enzymes In the first decade of the twenty-first century, enzymes and their multiple applications have played a critical role in the discovery and development of many new therapeutic agents.This book is a coordinated compilation of research expertise and current opinion uniquely focused on enzymes and their properties and applications. Compiled by editors with a combined pharmaceutical experience of over sixty years, the text provides in-depth reviews of recent developments in selective topics on biosynthesis, biocatalysis, and chemical biology of enzymes as it applies to drug discovery, development, and manufacture. The first in a multi-part series on enzymes, this volume features three sections: New Approaches to Find and Modify Enzymes describes the emerging field of metagenomics, presents the practical applications of directed evolution to enzymes and pathways, and explores approaches for the discovery and design of biocatalysts Biocatalytic Applications reviews specific applications of different reactions in producing active pharmaceutical ingredients and surveys recent developments employing enzymes in organic synthesis Biosynthetic Applications goes over successful drug discoveries and developments by combinatorial biosynthesis and reviews the application of combinatorial biosynthesis among multiple compatible hosts These timely discussions, which cover everything from chemical biology of enzymes, to the redesign of binding and catalytic specificities of enzymes, make this volume a valuable tool for keeping up to date. As such, it is an important read for researchers, students, and professors in the study of biotechnology, life sciences, biochemistry, enzymology, medicinal chemistry, and natural products.

An authoritative review of the latest developments in thechemical biology of enzymesIn the first decade of the twenty-first century, enzymes andtheir multiple applications have played a critical role in thediscovery and development of many new therapeutic agents.This bookis a coordinated compilation of research expertise and currentopinion uniquely focused on enzymes and their properties andapplications.Compiled by editors with a combined pharmaceutical experience ofover sixty years, the text provides in-depth reviews of recentdevelopments in selective topics on biosynthesis, biocatalysis, andchemical biology of enzymes as it applies to drug discoverydevelopment, and manufacture.The first in a multi-part series on enzymes, this volumefeatures three sections:* New Approaches to Find and Modify Enzymes describes the emergingfield of metagenomics, presents the practical applications ofdirected evolution to enzymes and pathways, and explores approachesfor the discovery and design of biocatalysts* Biocatalytic Applications reviews specific applications ofdifferent reactions in producing active pharmaceutical ingredientsand surveys recent developments employing enzymes in organicsynthesis* Biosynthetic Applications goes over successful drug discoveriesand developments by combinatorial biosynthesis and reviews theapplication of combinatorial biosynthesis among multiple compatiblehostsThese timely discussions, which cover everything from chemicalbiology of enzymes, to the redesign of binding and catalyticspecificities of enzymes, make this volume a valuable tool forkeeping up to date. As such, it is an important read forresearchers, students, and professors in the study ofbiotechnology, life sciences, biochemistry, enzymology, medicinalchemistry, and natural products.

Contributors.Preface.PART A NEW APPROACHES TO FINDING AND MODIFYINGENZYMES.1 Functional Metagenomics as a Technique for the Discovery ofNovel Enzymes and Natural Products (Luke A. Moe, Matthew D.McMahon, and Michael G. Thomas).2 Directed Enzyme and Pathway Evolution (Jacob Vick andClaudia Schmidt-Dannert).3 Combining Natural Biodiversity and Molecular-DirectedEvolution to Develop New Industrial Biocatalysts and Drugs(Laurent Fourage, Celine Ayrinhac, Johann Brot, ChristopheUllmann, Denis Wahler, and Jean-Marie Sonet).4 Principles of Enzyme Optimization for the Rapid Creation ofIndustrial Biocatalysts (Richard J. Fox and Lori Giver).PART B BIOCATALYTIC APPLICATIONS.5 Enzyme Catalysis in the Synthesis of Active PharmaceuticalIngredients (Animesh Goswami).6 Enzymatic Processes for the Production of PharmaceuticalIntermediates (David Rozzell and Jim Lalonde).7 Novel Developments Employing Redox Enzymes: Old Enzymes in NewClothes (Kurt Faber, Silvia M. Glueck, Birgit Seisser, andWolfgang Kroutil).PART C BIOSYNTHETIC APPLICATIONS.8 Drug Discovery and Development by Combinatorial Biosynthesis(Matthew A. DeSieno, Carl A. Denard, and Huimin Zhao).9 Reprogramming Daptomycin and A54145 Biosynthesis to ProduceNovel Lipopeptide Antibiotics (Richard H. Baltz, Kien T. Nguyenand Dylan C. Alexander).10 Pathway and Enzyme Engineering and Applications forGlycodiversification (Lishan Zhao and Hung-wen Liu).Index.