Medical Mycology

Medical Mycology: Cellular and Molecular techniques is a clear and concise overview of the subject that details the techniques essential for ongoing research in the area. Drawing together contributions from both scientists and clinicians working in the field, the text will provide a valuable perspective on the applicability of specific techniques to patient care. A wide range of molecular, immunological and cytological techniques are discussed throughout, with the inclusion of protocol section in each chapter designed to provide both a background a up-to-date account of the applications of each procedure. Every technique is fully referenced and illustrations are provided where required to enhance student understanding. comprehensive introduction to the key techniques critical to the study of medical mycology clear explanation of how each technique is applied in the lab contributions from internationally recognised experts in the field outlines the background to many techniques required for the successful completion of a research project An invaluable reference for students of microbiology, biochemistry and molecular biology as well as postgraduates and researchers in the field of medical mycology looking for an up-to-date overview of the latest laboratory techniques.

Medical Mycology: Cellular and Molecular techniques is aclear and concise overview of the subject that details thetechniques essential for ongoing research in the area. Drawingtogether contributions from both scientists and clinicians workingin the field, the text will provide a valuable perspective on theapplicability of specific techniques to patient care.A wide range of molecular, immunological and cytologicaltechniques are discussed throughout, with the inclusion of protocolsection in each chapter designed to provide both a background aup-to-date account of the applications of each procedure. Everytechnique is fully referenced and illustrations are provided whererequired to enhance student understanding.* comprehensive introduction to the key techniques critical tothe study of medical mycology* clear explanation of how each technique is applied in thelab* contributions from internationally recognised experts in thefield* outlines the background to many techniques required for thesuccessful completion of a research projectAn invaluable reference for students of microbiologybiochemistry and molecular biology as well as postgraduates andresearchers in the field of medical mycology looking for anup-to-date overview of the latest laboratory techniques.

Preface.List of Contributors.1 Diagnosis of Candida Infection in Tissue byImmunohistochemistry (Malcolm D. Richardson, Riina Rautemaa andJarkko Hietanen).2 Transmission Electron Microscopy of Pathogenic Fungi (GuyTronchin and Jean-Philippe Bouchara).3 Evaluation of Molecular Responses and Antifungal Activity ofPhagocytes to Opportunistic Fungi (Maria Simitsopoulou and EmmanuelRoilides).4 Determination of the Virulence Factors of Candida Albicans andRelated Yeast Species (Khaled H. Abu-Elteen and Mawieh Hamad).5 Analysis of Drug Resistance in Pathogenic Fungi (Gary P.Moran, Emmanuelle Pinjon, David C. Coleman and Derek J.Sullivan).6 Animal Models for Evaluation of Antifungal Efficacy AgainstFilamentous Fungi (Eric Dannaoui).7 Proteomic Analysis of Pathogenic Fungi (Alan Murphy).8 Extraction and Detection of DNA and RNA fromYeast (Patrick Geraghty and Kevin Kavanagh).9 Microarrays for Studying Pathogenicity in Candida Albicans(André Nantel, Tracey Rigby, Hervé´ Hogues andMalcolm Whiteway).10 Molecular Techniques for Application with AspergillusFumigatus (Nir Osherov and Jacob Romano).11 Promoter Analysis and Generation of Knock-out Mutants inAspergillus Fumigatus (Matthias Brock, Alexander Gehrke,Venelina Sugareva and Axel A. Brakhage).12 Microarray Technology for Studying the Virulence ofAspergillus Fumigatus (Darius Armstrong-James and ThomasRogers).13 Techniques and Strategies for Studying Virulence Factors inCryptococcus Neoformans (Nancy Lee and Guilhem Janbon).14 Genetic Manipulation of Zygomycetes (Ashraf S. Ibrahim andChristopher D. Skory).Index.