Rare-Earth Element Biochemistry: Characterization and Applications of Lanthanide-Binding Biomolecules

Rare-Earth Element Biochemistry: Characterization and Applications of Lanthanide-Binding Biomolecules, Volume 651 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Spectrophotometric methods to probe the solution chemistry of lanthanide complexes with macromolecules, Determination of affinities of lanthanide-binding proteins using chelator-buffered titrations, Electron Paramagnetic Resonance of Lanthanides, Characterization of lanthanoid binding proteins using NMR spectroscopy, Macromolecular crystallography for f-element complex characterization, Infrared spectroscopy probes ion binding geometries, Predicting lanthanide coordination structures in solution with molecular simulation, and much more.

Additional sections cover the Characteristics of Gd(III) spin labels for the study of protein conformations, Lanthanide-based resonance energy transfer biosensors for live-cell applications, Yttrium-86 PET imaging, Aqueous Chemistry of the Smallest Rare Earth: Comprehensive Characterization of Radioactive and Non-radioactive Scandium Complexes for Biological Applications, and In vitro selection and application of lanthanide-dependent DNAzymes.

1. Spectrophotometric methods to probe the solution chemistry of lanthanide complexes with macromolecules Gauthier Deblonde 2. Determination of affinities of lanthanide-binding proteins using chelator-buffered titrations Joseph A. Cotruvo Jr. 3. Electron Paramagnetic Resonance of Lanthanides Gareth R. Eaton 4. Characterization of lanthanoid binding proteins using NMR spectroscopy Claudio Luchinat and Giacomo Parigi 5. Macromolecular crystallography for f-element complex characterization Rebecca Abergel 6. Infrared spectroscopy probes ion binding geometries Carlos R. Baiz 7. Predicting lanthanide coordination structures in solution with molecular simulation David Cantu 8. Characteristics of Gd(III) spin labels for the study of protein conformations Daniella Goldfarb 9. Lanthanide-based resonance energy transfer biosensors for live-cell applications Lawrence W. Miller 10. Yttrium-86 PET imaging Peter Caravan and Mariane Le Fur 11. Aqueous Chemistry of the Smallest Rare Earth: Comprehensive Characterization of Radioactive and Non-radioactive Scandium Complexes for Biological Applications Eszter Boros 12. In vitro selection and application of lanthanide-dependent DNAzymes Juewen Liu