Beschreibung:
- Provides general guidelines and strategies for isolation and crystallization of membrane proteins- Gives detailed protocols that have wide application, and low specialized equipment needs- Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitopetagged proteins
I. Strategies and Techniques Purification Strategies for Membrane Proteins Chromatographic Techniques and Basic Operations in Membrane Protein Purification Production and purification of recombinant membrane proteins SDS Electrophoresis Techniques Blue-Native Electrophoresis Preparative Isoelectric Focussing Membrane protein crystallization