Super-Resolution Microscopy Techniques in the Neurosciences

Super-Resolution Microscopy Techniques in the Neurosciences serves as a comprehensive description of current super-resolution techniques, including the physical principles that allowed for their development, some of the most recent neurobiological applications and selected information for the practical use of these technologies. Written for the Neuromethods series, this detailed work contains contributions from experts in the field and provides key implementation advice to ensure successful results in the lab.

Authoritative and cutting-edge, Super-Resolution Microscopy Techniques in the Neurosciences is an ideal guide for researchers aiming to continue increasing the resolution in the imaging capabilities of neuroscientists and thereby changing the perspective in which cellular biology processes are understood at the nanometer scale.

Light Microscopy and Resolution.- Super-Resolution Microscopy: Principles, Techniques, and Applications.- Foundations of STED Microscopy.- Application of Real-Time STED Imaging to Synaptic Vesicle Motion.- Photoactivated Localization Microscopy for Cellular Imaging.- Data Analysis for Single-Molecule Localization Microscopy.- Super-Resolution Fluorescence Microscopy Using Structured Illumination.- Application of Three-Dimensional Structured Illumination Microscopy in Cell Biology: Pitfalls and Practical Considerations.- Scanning Near-Field Optical Microscopy for Investigations of Bio-Matter.- Atomic Force Microscopy of Living Cells.- X-Ray Microscopy for Neuroscience: Novel Opportunities by Coherent Optics.- Nonlinear Optics Approaches Towards Sub-Diffraction Resolution in CARS Imaging.- Photo-Oxidation Microscopy: Bridging the Gap Between Fluorescence and Electron Microscopy.- Requirements for Samples in Super-Resolution Fluorescence Microscopy.- Probing Biological Samples in High-Resolution Microscopy: Making Sense of Spots.