Synthetic Antibodies
Beschreibung:
Authoritative and practical, Synthetic Antibodies: Methods and Protocols is an ideal guide for scientists seeking to propel the vital study of antibody research.
Antibody Mimetics, Peptides, and Peptidomimetics.- Construction of a scFv Library with Synthetic, Non-Combinatorial CDR Diversity.- Enzymatic Assembly for scFv Library Construction.- Directed Evolution of Protein Thermal Stability Using Yeast Surface Display.- Whole Cell Panning with Phage Display.- Generating Conformation and Complex-Specific Synthetic Antibodies.- High-Throughput IgG Conversion of Phage Displayed Fab Antibody Fragments by AmplYFast.- Utilization of Selenocysteine for Site-Specific Antibody Conjugation.- Solubility Characterization andImaging of Intrabodies Using GFP-Fusions.- Antibody Validation by Immunoprecipitation Followed by Mass Spectrometry Analysis.- Novel HPLC-Based Screening Method to Assess Developability of Antibody-Like Molecules.- Glycosylation Profiling of /beta T Cell Receptor Constant Domains Expressed in Mammalian Cells.- A Proximity-Based Assay for Identification of Ligand and Membrane Protein Interaction in Living Cells.- A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteins.- Data-Driven Antibody Engineering Using Genedata Biologics(TM).- Selection of Aptamers Against Whole Living Cells: From Cell-SELEX to Identification of Biomarkers.- Rapid Selection of RNA Aptamers that Activate Fluorescence of Small Molecules.- An Enzyme-Linked Aptamer Sorbent Assay to Evaluate Aptamer Binding.- Incorporating Aptamers in the Multiple Analyte Profiling Assays (xMAP): Detection of C-Reactive Protein.- Transferring the Selectivity of a Natural Antibody into a Molecularly Imprinted Polymer.- Preparation of Molecularly Imprinted Microspheres by Precipitation Polymerization.- Generation of Janus Molecularly Imprinted Polymer Particles.- Surface Engineering of Nanoparticles to Create Synthetic Antibodies.- H5N1 Virus Plastic Antibody Based on Molecularly Imprinted Polymers.- Replacement of Antibodies in Pseudo-ELISAs: Molecularly Imprinted Nanoparticles for Vancomycin Detection.- Cell and Tissue Imaging with Molecularly Imprinted Polymers.