Ultra-High Performance Liquid Chromatography and Its Applications

Explores both the benefits and limitations of new UHPLC technology High performance liquid chromatography (HPLC) has been widely used in analytical chemistry and biochemistry to separate, identify, and quantify compounds for decades. The science of liquid chromatography, however, was revolutionized a few years ago with the advent of ultra-high performance liquid chromatography (UHPLC), which made it possible for researchers to analyze sample compounds with greater speed, resolution, and sensitivity. Ultra-High Performance Liquid Chromatography and Its Applications enables readers to maximize the performance of UHPLC as well as develop UHPLC methods tailored to their particular research needs. Readers familiar with HPLC methods will learn how to transfer these methods to a UHPLC platform and vice versa. In addition, the book explores a variety of UHPLC applications designed to support research in such fields as pharmaceuticals, food safety, clinical medicine, and environmental science. The book begins with discussions of UHPLC method development and method transfer between HPLC and UHPLC platforms. It then examines practical aspects of UHPLC. Next, the book covers: Coupling UHPLC with mass spectrometry Potential of shell particles in fast liquid chromatography Determination of abused drugs in human biological matrices Analyses of isoflavones and flavonoids Therapeutic protein characterization Analysis of illicit drugs The final chapter of the book explores the use of UHPLC in drug metabolism and pharmacokinetics studies for traditional Chinese medicine. With its frank discussions of UHPLC's benefits and limitations, Ultra-High Performance Liquid Chromatography and Its Applications equips analytical scientists with the skills and knowledge needed to take full advantage of this new separation technology.

Explores both the benefits and limitations of new UHPLCtechnologyHigh performance liquid chromatography (HPLC) has been widelyused in analytical chemistry and biochemistry to separateidentify, and quantify compounds for decades. The science of liquidchromatography, however, was revolutionized a few years ago withthe advent of ultra-high performance liquid chromatography (UHPLC)which made it possible for researchers to analyze sample compoundswith greater speed, resolution, and sensitivity.Ultra-High Performance Liquid Chromatography and ItsApplications enables readers to maximize the performance ofUHPLC as well as develop UHPLC methods tailored to their particularresearch needs. Readers familiar with HPLC methods will learn howto transfer these methods to a UHPLC platform and vice versa. Inaddition, the book explores a variety of UHPLC applicationsdesigned to support research in such fields as pharmaceuticalsfood safety, clinical medicine, and environmental science.The book begins with discussions of UHPLC method development andmethod transfer between HPLC and UHPLC platforms. It then examinespractical aspects of UHPLC. Next, the book covers:* Coupling UHPLC with mass spectrometry* Potential of shell particles in fast liquid chromatography* Determination of abused drugs in human biological matrices* Analyses of isoflavones and flavonoids* Therapeutic protein characterization* Analysis of illicit drugsThe final chapter of the book explores the use of UHPLC in drugmetabolism and pharmacokinetics studies for traditional Chinesemedicine.With its frank discussions of UHPLC's benefits and limitationsUltra-High Performance Liquid Chromatography and ItsApplications equips analytical scientists with the skills andknowledge needed to take full advantage of this new separationtechnology.

Preface viiContributors ix1. UHPLC Method Development 1Shujun Chen and Alireza Kord2. Method Transfer Between HPLC and UHPLC Platforms 31Gregory K. Webster, Thomas F. Cullen, and Laila Kott3. Practical Aspects of Ultrahigh Performance LiquidChromatography 55Naijun Wu, Christopher J. Welch, Theresa K. Natishan, Hong GaoTilak Chandrasekaran, and Li Zhang4. Coupling UHPLC with MS: The Needs, Challenges, andApplications 95Julie Schappler, Serge Rudaz, Jean-Luc Veuthey, and DavyGuillarme5. The Potential of Shell Particles in Fast LiquidChromatography 133Szabolcs Fekete and Jeno Fekete6. UHPLC Determination of Drugs of Abuse in Human BiologicalMatrices 169Fabio Gosetti, Eleonora Mazzucco, and Maria CarlaGennaro7. UHPLC in the Analyses of Isoflavones and Flavonoids 197Sylwia Magiera and Irena Baranowska8. UHPLC for Characterization of Protein Therapeutics 235Jennifer C. Rea, Yajun Jennifer Wang, and Taylor Zhang9. UHPLC/MS Analysis of Illicit Drugs 253Guifeng Jiang, Jason R. Stenzel, Ray Chen, and DiabElmashni10. Ultra-High Performance Liquid Chromatography - MassSpectrometry and Its Application 271Zhili Xiong, Ying Deng, and Famei LiIndex 291