Proteins in Solution and at Interfaces

Explores new applications emerging from our latest understanding of proteins in solution and at interfaces Proteins in solution and at interfaces increasingly serve as the starting point for exciting new applications, from biomimetic materials to nanoparticle patterning. This book surveys the state of the science in the field, offering investigators a current understanding of the characteristics of proteins in solution and at interfaces as well as the techniques used to study these characteristics. Moreover, the authors explore many of the new and emerging applications that have resulted from the most recent studies. Topics include protein and protein aggregate structure; computational and experimental techniques to study protein structure, aggregation, and adsorption; proteins in non-standard conditions; and applications in biotechnology. Proteins in Solution and at Interfaces is divided into two parts: Part One introduces concepts as well as theoretical and experimental techniques that are used to study protein systems, including X-ray crystallography, nuclear magnetic resonance, small angle scattering, and spectroscopic methods Part Two examines current and emerging applications, including nanomaterials, natural fibrous proteins, and biomolecular thermodynamics The book's twenty-three chapters have been contributed by leading experts in the field. These contributions are based on a thorough review of the latest peer-reviewed findings as well as the authors' own research experience. Chapters begin with a discussion of core concepts and then gradually build in complexity, concluding with a forecast of future developments. Readers will not only gain a current understanding of proteins in solution and at interfaces, but also will discover how theoretical and technical developments in the field can be translated into new applications in material design, genetic engineering, personalized medicine, drug delivery, biosensors, and biotechnology.

Explores new applications emerging from our latestunderstanding of proteins in solution and at interfacesProteins in solution and at interfaces increasingly serve as thestarting point for exciting new applications, from biomimeticmaterials to nanoparticle patterning. This book surveys the stateof the science in the field, offering investigators a currentunderstanding of the characteristics of proteins in solution and atinterfaces as well as the techniques used to study thesecharacteristics. Moreover, the authors explore many of the new andemerging applications that have resulted from the most recentstudies. Topics include protein and protein aggregate structure;computational and experimental techniques to study proteinstructure, aggregation, and adsorption; proteins in non-standardconditions; and applications in biotechnology.Proteins in Solution and at Interfaces is divided intotwo parts:* Part One introduces concepts as well as theoretical andexperimental techniques that are used to study protein systemsincluding X-ray crystallography, nuclear magnetic resonance, smallangle scattering, and spectroscopic methods* Part Two examines current and emerging applicationsincluding nanomaterials, natural fibrous proteins, and biomolecularthermodynamicsThe book's twenty-three chapters have been contributed byleading experts in the field. These contributions are based on athorough review of the latest peer-reviewed findings as well as theauthors' own research experience. Chapters begin with a discussionof core concepts and then gradually build in complexity, concludingwith a forecast of future developments.Readers will not only gain a current understanding of proteinsin solution and at interfaces, but also will discover howtheoretical and technical developments in the field can betranslated into new applications in material design, geneticengineering, personalized medicine, drug delivery, biosensors, andbiotechnology.

PREFACE ixCONTRIBUTORS xiiiPART I1 X-Ray Crystallography of Biological Macromolecules:Fundamentals and Applications 3Antonio L. Llamas-Saiz and Mark J. van Raaij2 Nuclear Magnetic Resonance Methods for Studying SolubleFibrous, and Membrane-Embedded Proteins 23Victoria A. Higman3 Small-Angle X-Ray Scattering Applied to Proteins in Solution49Leandro Ramos Souza Barbosa, Francesco Spinozzi, Paolo Marianiand Rosangela Itri4 Analyzing the Solution State of Protein StructureInteractions, and Ligands by Spectroscopic Methods 73Veronica I. Dodero and Paula V. Messina5 Resolving Membrane-Bound Protein Orientation and Conformationby Neutron Reflectivity 99Hirsh Nanda6 Investigating Protein Interactions at Solid Surfaces--InSitu, Nonlabeling Techniques 113Olof Svensson, Javier Sotres, and Alejandro Barrantes7 Calorimetric Methods to Characterize the Forces DrivingMacromolecular Association and Folding Processes 139Conceic¸ ~ao A.S.A. Minetti, Peter L. Privalov, andDavid P. Remeta8 Virtual Ligand Screening Against Comparative Models ofProteins 179Hao Fan9 Atomistic and Coarse-Grained Molecular Dynamics Simulations ofMembrane Proteins 193Thomas J. Piggot, Peter J. Bond, and Syma KhalidPART II10 Preparation of Nanomaterials Based on Peptides and Proteins209Yujing Sun and Zhuang Li11 Natural Fibrous Proteins: Structural Analysis, Assembly, andApplications 219Mark J. van Raaij and Anna Mitraki12 Amyloid-Like Fibrils: Origin, Structure, Properties, andPotential Technological Applications 233Pablo Taboada, Silvia Barbosa, Josue Juarez, Manuel-AlatorreMeda, and Vyctor Mosquera13 Proteins and Peptides in Biomimetic Polymeric Membranes283Alfredo Gonzalez-Perez14 Study of Proteins and Peptides at Interfaces By MolecularDynamics Simulation Techniques 291David Poger and Alan E. Mark15 A Single-Molecule Approach to Explore the Role of the SolventEnvironment in Protein Folding 315Katarzyna Tych and Lorna Dougan16 Enhanced Functionality of Peroxidases By Its Immobilizationat The Solid-Liquid Interface of Mesoporous Materials andNanoparticles 335Jose Campos-Teran, Iker Inarritu, Jorge Aburto, and EduardoTorres17 Superactivity of Enzymes in Supramolecular Hydrogels353Ye Zhang and Bing Xu18 Surfactant Proteins and Natural Biofoams 365Malcolm W. Kennedy and Alan Cooper19 Promiscuous Enzymes 379Luis F. Olguin20 Thermodynamics and Kinetics of Mixed Protein/SurfactantAdsorption Layers at Liquid Interfaces 389Reinhard Miller, E.V. Aksenenko, V.S. Alahverdjieva, V.B.Fainerman, C.S. Kotsmar, J. Kragel, M.E. Leser, J.Maldonado-Valderrama, V. Pradines, C. Stefaniu, A. Stocco, and R.Wustneck21 Application of Force Spectroscopy Methods to the Study ofBiomaterials 429Chuan Xu and Erika F. Merschrod S.22 Protein Gel Rheology 437Katie Weigandt and Danilo Pozzo23 Exploring Biomolecular Thermodynamics in Aqueous andNonaqueous Environments using Time-Resolved Photothermal Methods449Randy W. Larsen, Carissa M. Vetromile, William A. Maza, KhoaPham, Jaroslava MiksovskaINDEX 473