Animal Cell Culture

This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

This is a comprehensive research guide that describes both the keynew techniques and more established methods. Every chapterdiscusses the merits and limitations of the various approaches andthen provides selected tried-and-tested protocols, as well as aplethora of good practical advice, for immediate use at the bench.It presents the most accessible and comprehensive introductionavailable to the culture and experimental manipulation of animalcells. Detailed protocols for a wide variety of methods provide thecore of each chapter, making new methodology easilyaccessible.This book is an essential laboratory manual for all undergraduatesand graduates about to embark on a cell culture project. It is abook which both experienced researchers and those new to the fieldwill find invaluable.

Contributors.Preface.Abbreviations.Protocols.1 The Cell Culture Laboratory (Sue Clarke and JanetteDillon).2 Sterilization (Peter L. Roberts).3 Microscopy of Living Cells (Colin Gray and DanielZicha).4 Basic Techniques and Media, the Maintenance of Cell Lines, andSafety (John M. Davis).5 Development and Optimization of Serum- and Protein-freeCulture Media (Stephen F. Gorfien and David W. Jayme).6 Cryopreservation and Banking of Cell Lines (Glyn N. StaceyRoss Hawkins and Roland A. Fleck).7 Primary Culture of Specific Cell Types and the Establishmentof Cell Lines (Kee Woei Ng, Mohan Chothirakottu Abraham, DavidTai Wei Leong, Chris Morris and Jan-Thorsten Schantz).8 Cloning (John Clarke, Alison Porter and John M.Davis).9 The Quality Control of Animal Cell Lines and the PreventionDetection and Cure of Contamination (Peter Thraves and CathyRowe).10 Systems for Cell Culture Scale-up (Jennifer Halsall andJohn M. Davis).11 Good Laboratory Practice in the Cell Culture Laboratory(Barbara Orton).Index.Colour plates.